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Product Introduction

Bioactivity

Description	α-Vitamin E (Dexrabeprazole Sodium) is a naturally-occurring form of vitamin E, a fat-soluble vitamin with potent antioxidant properties.
Targets&IC50	ERα (mouse):> 500 nM (EC50), Erythrocyte:3.658 μg/mL, J774 cells:> 500 μM, MCF7 cells:> 300 μM, THP-1 cells:> 500 μM, RPMI-8226 cells:> 300 μM, MT4 cells:165 μM, U-937 cells:100 μM, HepG2 cells:> 300 μM, HL-60 cells:255 μM
In vitro	vitamin E is capable of inducing necrosis in TC-1 cells and has antioxidant effects against nitric oxide[1].
In vivo	Vitamin E induces TC-1 cell necrosis in vivo and reduces tumor volume in TC-1 tumor-bearing mice. Vitamin E reduces myeloid derived suppressor cells(MDSCs) in tumors of TC-1 tumor-bearing mice and enhances T cell accumulation[1].
Cell Research	For in vitro cytotoxicity experiments, 1×105 TC-1 cells per well are added to 24-well plates. Eighteen hours later, tumor cells are treated with Vitamin E (0, 25, 50 μM). After 18 hours, apoptotic (Annexin V+ and 7AAD−) and necrotic (Annexin V+ and 7AAD+) cells are measured using PE Annexin V Apoptosis Detection Kit I. (Only for Reference)
Alias	Vitamin E, D-α-Tocopherol, Alpha-Tocopherol, 5,7,8-Trimethyltocol, (+)-alpha-Tocopherol

Chemical Properties

Molecular Weight	430.71
Formula	C₂₉H₅₀O₂
Cas No.	59-02-9
Smiles	CC1=C2C(CC[C@](CCC[C@@H](CCC[C@@H](CCCC(C)C)C)C)(C)O2)=C(C)C(O)=C1C
Relative Density.	0.95g/mLat 25°C(lit.)

Storage & Solubility Information

Storage

Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 12 mg/mL (27.86 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1.2 mg/mL (2.79 mM), Solution.

Saline: 25 mg/mL (58.04 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3217 mL	11.6087 mL	23.2175 mL	116.0874 mL
5 mM	0.4643 mL	2.3217 mL	4.6435 mL	23.2175 mL
10 mM	0.2322 mL	1.1609 mL	2.3217 mL	11.6087 mL
20 mM	0.1161 mL	0.5804 mL	1.1609 mL	5.8044 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Sci Citations

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc