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Rotenone

Rotenone
Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.
Catalog No. T2970Cas No. 83-79-4
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Purity:99.48%
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Rotenone

Catalog No. T2970Cas No. 83-79-4

Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
500 mg$42In Stock
1 g$50In Stock
5 g$89In Stock
1 mL x 10 mM (in DMSO)$46In Stock
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Product Introduction

Bioactivity
Description
Rotenone (Rotocide) is a natural product and a botanical insecticide. Rotenone is a mitochondrial electron transport complex I inhibitor that promotes the production of reactive oxygen species in mitochondria and induces apoptosis.
In vitro
METHODS: Human leukemia cells HL-60 were treated with Rotenone (0-1000 nM) for 30 min and oxygen consumption was measured using a Clark oxygen electrode.
RESULTS: Rotenone dose-dependently inhibited HL-60 cell respiration. Rotenone inhibited cellular respiration by more than 96% at 500 nM. [1]
METHODS: 10th DIV cells were treated with Rotenone (20 nM) for 48 h. LDH levels were measured by LDH release assay.
RESULTS: LDH release can be used as an indicator of general cytotoxicity. rotenone induced an increase in LDH release, and LDH activity increased more than 6-fold compared to the control group. LDH activity increased more than 6-fold compared to the control group.[2]
In vivo
METHODS: To establish a reproducible mouse model of Rotenone-induced Parkinson's disease (PD), Rotenone (2.5 mg/kg) was administered intraperitoneally to C57Bl/6 mice once daily for two weeks.
RESULTS: Systemic exposure of mice to Rotenone resulted in progressive accumulation and regional spread of p129 aggregates, which preceded the maximal loss of DAn. [3]
METHODS: To study neurotoxicity, Rotenone (30-100 mg/kg) was administered orally to C57BL/6 mice once daily for fifty-six days.
RESULTS: The survival of Rotenone-treated mice at 30 mg/kg was unchanged from 28-56 days, although the survival of Rotenone-treated mice was reduced to approximately 70% within one week. 100 mg/kg Rotenone-treated mice showed a sudden decrease in survival after 28 days, and ultimately to approximately 15% after 56 days. A regimen of Rotenone given chronically at 30 mg/kg for 56 days is more useful for understanding the mechanisms of DA neurodegeneration. [4]
AliasRotenon, Paraderil, Barbasco, Rotocide, Dactinol
Chemical Properties
Molecular Weight394.42
FormulaC23H22O6
Cas No.83-79-4
Storage & Solubility Information
Storagekeep away from direct sunlight,store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 18.33 mg/mL (46.48 mM), Sonication is recommended.
10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.94 mg/mL (9.99 mM), Suspension. Working solution is recommended to be prepared and used immediately.
Solution Preparation Table
10% DMSO+40% PEG300+5% Tween 80+45% Saline/DMSO
1mg5mg10mg50mg
1 mM2.5354 mL12.6768 mL25.3537 mL126.7684 mL
5 mM0.5071 mL2.5354 mL5.0707 mL25.3537 mL
DMSO
1mg5mg10mg50mg
10 mM0.2535 mL1.2677 mL2.5354 mL12.6768 mL
20 mM0.1268 mL0.6338 mL1.2677 mL6.3384 mL

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