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N-tert-butyl-α-Phenylnitrone (Alias: (Z)-N-benzylidene-2-Methylpropan-2-aMine oxide)

Name: N-tert-butyl-α-Phenylnitrone
Brand: TargetMol
SKU: T23046
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

Catalog No. T23046 Copy Product Info

Purity: 99.84%

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N-tert-butyl-α-Phenylnitrone ((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) inhibits COX2 catalytic activity. N-tert-butyl-α-Phenylnitrone((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) possesses potent reactive oxygen species scavenging, anti-inflammatory, neuroprotective, anti-aging, and anti-diabetic activities, and can penetrate the blood-brain barrier.

N-tert-butyl-α-Phenylnitrone

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Catalog No. T23046

Alias (Z)-N-benzylidene-2-Methylpropan-2-aMine oxide

Cas No. 3376-24-7

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Pack Size	Price	USA Stock	Global Stock
50 mg	$37	In Stock	In Stock
100 mg	$52	In Stock	In Stock
200 mg	$78	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

In stock · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.84%

Color:White to Yellow

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	N-tert-butyl-α-Phenylnitrone ((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) inhibits COX2 catalytic activity. N-tert-butyl-α-Phenylnitrone((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) possesses potent reactive oxygen species scavenging, anti-inflammatory, neuroprotective, anti-aging, and anti-diabetic activities, and can penetrate the blood-brain barrier.
In vitro	Treatment of 25-100 μM N-tert-butyl-α-Phenylnitrone significantly reduces 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced intracellular ROS accumulation. N-tert-butyl-α-Phenylnitrone also attenuates AAPH-induced cytotoxicity, matrix degradation, and apoptosis, inhibiting AAPH-induced ERK/MAPK pathway activation[1].
In vivo	In C57Bl/6 mice induced by lipopolysaccharide (LPS), intraperitoneal injection of 100 mg/kg N-tert-butyl-α-Phenylnitrone twice a day (on gestational day 8) abolishes LPS-induced lipid peroxidation, nitrate tyrosine residue levels and GSH depletion, and reduces the incidence of external malformations[2].
Synonyms	(Z)-N-benzylidene-2-Methylpropan-2-aMine oxide

Chemical Properties

Molecular Weight	177.24
Formula	C₁₁H₁₅NO
Cas No.	3376-24-7
Smiles	CC(C)(C)[N+]([O-])=CC1=CC=CC=C1
Relative Density.	0.990 g/cm3

Storage & Solubility Information

Storage

keep away from moisture,store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (282.1 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (11.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.6421 mL	28.2103 mL	56.4207 mL	282.1034 mL
5 mM	1.1284 mL	5.6421 mL	11.2841 mL	56.4207 mL
10 mM	0.5642 mL	2.8210 mL	5.6421 mL	28.2103 mL
20 mM	0.2821 mL	1.4105 mL	2.8210 mL	14.1052 mL
50 mM	0.1128 mL	0.5642 mL	1.1284 mL	5.6421 mL
100 mM	0.0564 mL	0.2821 mL	0.5642 mL	2.8210 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc