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N-tert-butyl-α-Phenylnitrone

Name: N-tert-butyl-α-Phenylnitrone
Brand: TargetMol
SKU: T23046
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

🥰Excellent

Catalog No. T23046Cas No. 3376-24-7

Alias (Z)-N-benzylidene-2-Methylpropan-2-aMine oxide

N-tert-butyl-α-Phenylnitrone ((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) inhibits COX2 catalytic activity. N-tert-butyl-α-Phenylnitrone((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) possesses potent reactive oxygen species scavenging, anti-inflammatory, neuroprotective, anti-aging, and anti-diabetic activities, and can penetrate the blood-brain barrier.

N-tert-butyl-α-Phenylnitrone

🥰Excellent

Purity: 99.84%

Catalog No. T23046Alias (Z)-N-benzylidene-2-Methylpropan-2-aMine oxideCas No. 3376-24-7

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$37	In Stock	In Stock
100 mg	$52	In Stock	In Stock
200 mg	$78	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$50	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.84%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	N-tert-butyl-α-Phenylnitrone ((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) inhibits COX2 catalytic activity. N-tert-butyl-α-Phenylnitrone((Z)-N-benzylidene-2-Methylpropan-2-aMine oxide) possesses potent reactive oxygen species scavenging, anti-inflammatory, neuroprotective, anti-aging, and anti-diabetic activities, and can penetrate the blood-brain barrier.
In vitro	Treatment of 25-100 μM N-tert-butyl-α-Phenylnitrone significantly reduces 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH)-induced intracellular ROS accumulation. N-tert-butyl-α-Phenylnitrone also attenuates AAPH-induced cytotoxicity, matrix degradation, and apoptosis, inhibiting AAPH-induced ERK/MAPK pathway activation[1].
In vivo	In C57Bl/6 mice induced by lipopolysaccharide (LPS), intraperitoneal injection of 100 mg/kg N-tert-butyl-α-Phenylnitrone twice a day (on gestational day 8) abolishes LPS-induced lipid peroxidation, nitrate tyrosine residue levels and GSH depletion, and reduces the incidence of external malformations[2].
Synonyms	(Z)-N-benzylidene-2-Methylpropan-2-aMine oxide

Chemical Properties

Molecular Weight	177.24
Formula	C₁₁H₁₅NO
Cas No.	3376-24-7
Smiles	CC(C)(C)[N+]([O-])=CC1=CC=CC=C1
Relative Density.	0.990 g/cm3
Color	Yellow
Appearance	Solid

Storage & Solubility Information

Storage

keep away from moisture,store at low temperature | Pure form: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (282.1 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (11.28 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	5.6421 mL	28.2103 mL	56.4207 mL	282.1034 mL
5 mM	1.1284 mL	5.6421 mL	11.2841 mL	56.4207 mL
10 mM	0.5642 mL	2.8210 mL	5.6421 mL	28.2103 mL
20 mM	0.2821 mL	1.4105 mL	2.8210 mL	14.1052 mL
50 mM	0.1128 mL	0.5642 mL	1.1284 mL	5.6421 mL
100 mM	0.0564 mL	0.2821 mL	0.5642 mL	2.8210 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc