Anti-ROS1 Polyclonal Antibody is a Rabbit antibody targeting ROS1. Anti-ROS1 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Rat

1. Sample:
Lane 1: Cerebrum (Rat) Lysate at 40 μg
Lane 2: Cerebellum (Rat) Lysate at 40 μg
Lane 3: Pituitary (Rat) Lysate at 40 μg
Lane 4: Siha (Human) Cell Lysate at 30 μg
Lane 5: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-ROS1 (TMAB-01635) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 258 kDa
Observed band size: >250/110 kDa
2. Blank control: THP-1. Primary Antibody (green line): Rabbit Anti-ROS1 antibody (TMAB-01635)
Dilution: 1 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 1ug/Test

Polyclonal

KLH conjugated synthetic peptide: human ROS1

Human

Growth factors,Proto-oncogenes,Receptor Tyrosine Kinases