Shopping Cart

Your shopping cart is currently empty

Leptomycin B

Name: Leptomycin B
Brand: TargetMol
SKU: T15735
Price: 213 USD
Availability: InStock
Rating: 5 (10 reviews)

Copy Product Info

😃Good

Hot

Catalog No. T15735Cas No. 87081-35-4

Alias LMB, CI 940

Leptomycin B (LMB) is a potent inhibitor of the nuclear export of proteins and is a potent antifungal antibiotic blocking the eukaryotic cell cycle. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue.

Leptomycin B

Copy Product Info

😃Good

Hot

Purity: 99.04%

Catalog No. T15735Alias LMB, CI 940Cas No. 87081-35-4

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$213	In Stock	In Stock
2 mg	$359	In Stock	In Stock
5 mg	$652	In Stock	In Stock
10 mg	$928	In Stock	In Stock
25 mg	$1,390	In Stock	In Stock
50 mg	$1,870	In Stock	In Stock
100 mg	$2,530	-	In Stock
1 mL x 10 mM (in DMSO)	$297	In Stock	In Stock

Add to Cart

Add to Quotation

In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Questions

Batch Information

Select Batch

Purity:99.04%

Resource Download

COA

Product Introduction

Leptomycin B AI Summary

Leptomycin B exhibits significant antiproliferative activity against human PC3 cells, demonstrating a fold change of 0.262 at a concentration of 10 ng/ml after 120 hours as determined by the MTT assay relative to DMSO. It effectively inhibits HIV1 Rev in human HeLa cells co-expressing renilla luciferase and firefly luciferase reporter genes, with an EC50 of 8.22 nM for renilla luciferase expression inhibition and a TC50 of 0.02 µM for firefly luciferase expression inhibition after 24 hours. Additionally, Leptomycin B inhibits CRM1-mediated nucleocytoplasmic transport in human HeLa cells with an IC50 of 1.0 nM, according to immunofluorescence assay results. This compound also exhibits antiviral activity against Influenza A virus, with an EC50 of 180.0 nM and a selectivity ratio greater than 4.0 due to a cytotoxicity CC50 greater than 880.0 nM in MDCK cells. Furthermore, Leptomycin B inhibits the fluorescently labeled PKI phi(0)Leu NES binding to human CRM1 expressed in E. coli BL21 (DE3) with an IC50 of 151.0 nM via a fluorescence polarization competition binding assay. It fully inhibits the immobilized HsCRM1-HsRanGTP-HsSPN binding to C-terminal His6-tagged and MBP-fused full-length human CRM1 expressed in E. coli SG13009 during a 20-minute pull-down assay at a concentration of 20 nmol..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Leptomycin B (LMB) is a potent inhibitor of the nuclear export of proteins and is a potent antifungal antibiotic blocking the eukaryotic cell cycle. Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a cysteine residue.
Synonyms	LMB, CI 940

Chemical Properties

Molecular Weight	540.73
Formula	C₃₃H₄₈O₆
Cas No.	87081-35-4
Smiles	CC\C(\C=C\[C@H]1OC(=O)C=C[C@@H]1C)=C\[C@H](C)C\C=C\C(\C)=C\[C@@H](C)C(=O)[C@@H](C)[C@H](O)[C@@H](C)C\C(C)=C\C(O)=O
Relative Density.	1.072 g/cm3 (Predicted)

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 101 mg/mL (186.78 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (7.4 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	1.8494 mL	9.2468 mL	18.4935 mL	92.4676 mL
5 mM	0.3699 mL	1.8494 mL	3.6987 mL	18.4935 mL
10 mM	0.1849 mL	0.9247 mL	1.8494 mL	9.2468 mL
20 mM	0.0925 mL	0.4623 mL	0.9247 mL	4.6234 mL
50 mM	0.0370 mL	0.1849 mL	0.3699 mL	1.8494 mL
100 mM	0.0185 mL	0.0925 mL	0.1849 mL	0.9247 mL

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc