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Indomethacin

Name: Indomethacin
Brand: TargetMol
SKU: T0458
Price: 37 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0458Cas No. 53-86-1

Alias Indomethacine, Indometacine, Indometacin, Indocin

Indomethacin is an orally active nonsteroidal anti-inflammatory drug (NSAID) that non-selectively inhibits cyclooxygenase enzymes COX-1 and COX-2, with IC₅₀ values of 18 nM and 26 nM, respectively. It exhibits good blood-brain barrier permeability and shows significant anti-inflammatory, anti-tumor, and anti-infective activities. Indomethacin is widely used in research related to cancer, inflammation, and viral infections, and is also commonly used to induce gastric ulcer models in animals.

Indomethacin

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Purity: 99.76%

Catalog No. T0458Alias Indomethacine, Indometacine, Indometacin, IndocinCas No. 53-86-1

Pack Size	Price	USA Warehouse	Global Warehouse
500 mg	$37	In Stock	In Stock
1 g	$50	In Stock	In Stock
5 g	$86	Inquiry	Inquiry
10 g	$126	Inquiry	Inquiry
1 mL x 10 mM (in DMSO)	$48	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.76%

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Indomethacin is an orally active nonsteroidal anti-inflammatory drug (NSAID) that non-selectively inhibits cyclooxygenase enzymes COX-1 and COX-2, with IC₅₀ values of 18 nM and 26 nM, respectively. It exhibits good blood-brain barrier permeability and shows significant anti-inflammatory, anti-tumor, and anti-infective activities. Indomethacin is widely used in research related to cancer, inflammation, and viral infections, and is also commonly used to induce gastric ulcer models in animals.
Targets&IC50	COX-2:14 μM, COX-1:0.28 μM
In vitro	METHODS: Human gastric cancer cells AGS were treated with Indomethacin (100-500 µM) for 24 h, and the expression levels of target proteins were detected by Western Blot. RESULTS: Indomethacin increased LC3-II protein levels in AGS cells, which may be a result of induction of autophagy or inhibition of lysosome-dependent autophagic degradation. [1] METHODS: Human tumor cells HT29 and A431 were treated with Indomethacin (1-10 µM) and EGF (25 ng/mL) for 24-48 h. Cell migration was detected by wound-healing assay. RESULTS: Indomethacin inhibited HT29 cell migration. After treating A431 cells with EGF only, the wound closed within 24 h, but the wound remained open after 24 h of treatment with Indomethacin. [2]
In vivo	METHODS: To test the antitumor activity in vivo, Indomethacin (1 mg/kg) was administered by gavage to CDF1 mice bearing Colon 26 xenografts twice daily for 7-21 days. RESULTS: Mean tumor weight was significantly lower in both the long-term and short-term Indomethacin administration groups, serum immunosuppressive acidic protein levels were significantly lower, serum IL-6 levels were also significantly lower, and prostaglandin E2 levels in tumor tissues were significantly lower than those in the control group. [3]
Disease Modeling Protocol	Small intestine inflammation model Modeling Mechanism： Indomethacin inhibits cyclooxygenase (COX) activity, reduces prostaglandin (PGE) synthesis, and disrupts the small intestinal mucosal repair function. Simultaneously, it damages tight junction proteins, increases intestinal permeability, and triggers local inflammation mediated by gut microbiota and intestinal contents, inducing the release of pro-inflammatory factors such as TNF-α and IL-6. Furthermore, it induces oxidative stress, reduces glutathione (GSH) and antioxidant enzyme (SOD, GPX) activity, and increases lipid peroxidation products (MDA) and total oxygen saturation (TOS), ultimately leading to small intestinal villus structure destruction, erosion, ulceration, and inflammatory infiltration. Related Products： Indomethacin (T0458) Modeling Method： Experimental Subject： Rats, Wistar, Female, 15 weeks old, Body weight 160–200 g Dosage and Administration Route： 25 mg/kg indomethacin, dissolved in 0.9% saline, administered via gastric lavage Dosing Frequency and Duration Model： Single dose Validation： The small intestinal tissue showed disordered villus structure, erosion and ulceration, and inflammatory cell infiltration, with a significantly elevated total pathological damage score; the levels of TNF-α and IL-6 in the small intestinal tissue were significantly elevated; the content of GSH and the activities of SOD and GPX were decreased, while the levels of MDA and TOS were increased; and the total antioxidant level (TAS) of the tissue was decreased. Precautions： All rats were anesthetized and sacrificed 6 hours after modeling, and the small intestine tissue was rapidly separated. References：Secgin S, et,al. Thiamine pyrophosphate may protect indomethacin-induced small intestinal enteropathy in rats by inhibiting intestinal inflammation and oxidative stress. Naunyn Schmiedebergs Arch Pharmacol. 2025 Oct;398(10):14441-14450. Gastric ulcer model Modeling Mechanism： Indomethacin inhibits the synthesis of COX-1/COX-2 prostaglandins, leading to decreased gastric mucosal defense, increased inflammation and oxidative stress, and thus causing gastric mucosal damage and ulcer formation. Related Products： Indomethacin (T0458) Modeling Method： Experimental Subject： Rats, Wistar, Equal numbers of males and females, 200–220 g Dosage and Administration Route： 30 mg/kg, dissolved in distilled water and administered via gavage Dosing Frequency and Duration Model： Single dose Validation： Serum EGF and PGE2 concentrations were significantly decreased. Immunohistochemical results showed that the expression levels of COX-1 and COX-2 in the gastric mucosa were significantly downregulated, and there was significant gastric mucosal epithelial shedding, edema, and inflammatory cell infiltration. The gastric mucosal injury index was significantly increased. Precautions： Before administration to all animals 24 hFasting was implemented. In the prevention experiment, animals were sacrificed 6 hours after gavage administration of indomethacin; in the treatment experiment, animals were administered the drug for 3 days after modeling, followed by a 24-hour fast before being sacrificed. References：Fang YF,et,al.Effect of Hydrotalcite on Indometacin-Induced Gastric Injury in Rats. Biomed Res Int. 2019 Apr 11;2019:4605748.
Synonyms	Indomethacine, Indometacine, Indometacin, Indocin

Chemical Properties

Molecular Weight	357.79
Formula	C₁₉H₁₆ClNO₄
Cas No.	53-86-1
Smiles	COC1=CC2=C(C=C1)N(C(=O)C1=CC=C(Cl)C=C1)C(C)=C2CC(O)=O
Relative Density.	1.32g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 104 mg/mL (290.67 mM), Sonication is recommended.

Ethanol: 17.9 mg/mL (50.03 mM), Heating is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.58 mg/mL (10.01 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.7949 mL	13.9747 mL	27.9494 mL	139.7468 mL
5 mM	0.5590 mL	2.7949 mL	5.5899 mL	27.9494 mL
10 mM	0.2795 mL	1.3975 mL	2.7949 mL	13.9747 mL
20 mM	0.1397 mL	0.6987 mL	1.3975 mL	6.9873 mL
50 mM	0.0559 mL	0.2795 mL	0.5590 mL	2.7949 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0279 mL	0.1397 mL	0.2795 mL	1.3975 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc