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Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA Protein (His), Biotinylated
(Synonyms: Harvey rat sarcoma viral oncogene homolog) Copy Product InfoSynonyms: Harvey rat sarcoma viral oncogene homolog
Catalog No. TMPY-05527 Copy Product Info
Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA Protein (His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59 kDa and the accession number is C3W5S1.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 μg | $172 | 7-10 days | 7-10 days | |
| 10 μg | $298 | 7-10 days | 7-10 days | |
| 20 μg | $577 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| Description | Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA Protein (His), Biotinylated is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59 kDa and the accession number is C3W5S1. |
| Species | H1N1 |
| Expression System | Baculovirus Insect Cells |
| Tag | C-His |
| Accession Number | C3W5S1 |
| Construction | A DNA sequence encoding the extracellular domain of Influenza A virus (A/California/04/2009 (H1N1)) (ACP41105.1) hemagglutinin (Met1-Gln529) (Native, HA1+HA2, uncleaved) was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro. Predicted N terminal: Asp 18 |
| Protein Purity | > 95 % as determined by SDS-PAGE. |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
| Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Synonyms | Harvey rat sarcoma viral oncogene homolog |
| Research Background | The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity. |
| Molecular Weight | 59 kDa (predicted) |
| Storage | Lyophilized powder: -20~-80°C for 1 year | Solution: -80°C for 6 months |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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Related Tags: Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA Protein (His), Biotinylated chemical structure | Influenza A H1N1 (A/California/04/2009) Hemagglutinin/HA Protein (His), Biotinylated molecular weight
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