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Isoprenaline hydrochloride

Catalog No. T1056   CAS 51-30-9
Synonyms: NCI-c55630, Isoproterenol hydrochloride, Isoprenaline HCl

Isoprenaline hydrochloride (NCI-c55630) is a potent beta-adrenergic agonist with the peripheral vasodilator, bronchodilator, and cardiac stimulating properties.

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Isoprenaline hydrochloride Chemical Structure
Isoprenaline hydrochloride, CAS 51-30-9
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1 g In stock $ 33.00
1 mL * 10 mM (in DMSO) In stock $ 30.00
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Purity: 100%
Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Isoprenaline hydrochloride (NCI-c55630) is a potent beta-adrenergic agonist with the peripheral vasodilator, bronchodilator, and cardiac stimulating properties.
In vitro Incubation of intact rat fat cells with isoprenaline (300 nM, 3 min) increased particulate cGMP- and cilostamide-inhibited, low-Km cAMP phosphodiesterase (cAMP-PDE) activity by about 50% and 100%, respectively [1]. Relaxation induced by isoprenaline was also potentiated by the cyclic GMP-inhibited PDE (PDE 3) inhibitor cilostamide (100 nM). Isoprenaline (5 nM and 10 microM) increased cyclic AMP levels and this effect was potentiated by cilostamide (10 microM), by rolipram, a cyclic AMP-specific PDE (PDE 4) inhibitor (10 microM) and by cyclic GMP-elevating agents (50 nM ANF or 30 nM SNP plus 100 nM DMPPO) [2]. Isoprenaline increased the phosphorylation levels of Akt, and the downstream FoxO1, FoxO3a, and CREB. When catecholamine binding to β-adrenoceptors, the G protein–coupled receptor kinase-2 (GRK2) mediates the translocation of PI3K to β-adrenoceptors and then enhances the recruitment of β-arrestin and AP-2, which finally results in the internalization and downregulation of β-adrenoceptors. It has reported that disrupts the interaction between PI3K and GRK2 by displacing class I PI3K isoforms blocks agonist-stimulated β-adrenoceptors internalization [3].
Cell Research Cells are seeded in 24-well culture dishes at a density of 2 to 5×10^4 cells per well. Experiments are performed after 3 to 5 days in culture when cells has reached confluence. Culture medium is aspirated and replaced by 0.5 mL of PBS containing the pharmacological agents. Treatments are performed in quadruplicate at 37°C. The type 3, 4 and 5 PDE inhibitors cilostamide (10 gM), rolipram (10 pM) and DMPPO (10 gM), respectively, are incubated with cells for 30 min before addition of adenylate or guanylate cyclase activators. Cyclic GMP and cyclic AMP are respectively increased in RASMC by stimulation of particulate guanylate cyclase with ANF (50 nM for 10 min) or fl-adrenoceptors with isoprenaline (5 nm for 5 min). At the end of the incubation period, the medium is removed and intracellular cyclic nucleotides are extracted by two ethanolic (65%) ishes at 4°C for 5 min. Ethanolic extracts are pooled, evaporated to dryness by a Speed-Vac system. The dried extract is dissolved in a suitable amount of assay buffer and cyclic nucleotide levels are measured by scintillation proximity assay [3].
Synonyms NCI-c55630, Isoproterenol hydrochloride, Isoprenaline HCl
Molecular Weight 247.719
Formula C11H18ClNO3
CAS No. 51-30-9

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

H2O: 201.8 mM

DMSO: 201.8 mM

TargetMolReferences and Literature

1. Degerman E, et al. Evidence that insulin and isoprenaline activate the cGMP-inhibited low-Km cAMP phosphodiesterase in rat fat cells by phosphorylation. Proc Natl Acad Sci U S A. 1990 Jan;87(2):533-7 2. Delpy E, et al. Effects of cyclic GMP elevation on isoprenaline-induced increase in cyclic AMP and relaxation in rat aortic smooth muscle: role of phosphodiesterase 3. Br J Pharmacol. 1996 Oct;119(3):471-8. 3. Qian W, et al. Wogonin Attenuates Isoprenaline-Induced Myocardial Hypertrophy in Mice by Suppressing the PI3K/Akt Pathway. Front Pharmacol. 2018 Aug 13;9:896.

TargetMolCitations

1. Cai S, Chang J, Su M, et al.miR-455-5p promotes pathological cardiac remodeling via suppression of PRMT1-mediated Notch signaling pathway.Cellular and Molecular Life Sciences.2023, 80(12): 359.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Drug Library Anti-Cancer Approved Drug Library Anti-Neurodegenerative Disease Compound Library Human Metabolite Library Anti-Colorectal Cancer Compound Library Glycometabolism Compound Library Antioxidant Compound Library Reprogramming Compound Library Anti-Cancer Compound Library Bioactive Lipid Compound Library

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Keywords

Isoprenaline hydrochloride 51-30-9 GPCR/G Protein Metabolism Neuroscience PI3K/Akt/mTOR signaling PI3K Endogenous Metabolite Adrenergic Receptor PDE peripheral vasodilator Isoprenaline NCI-c55630 Beta Receptor cardiac stimulating activities Inhibitor Isoproterenol Isoproterenol hydrochloride Isoprenaline Hydrochloride inhibit bronchodilator Isoprenaline HCl Isoproterenol Hydrochloride inhibitor

 

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