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5'-Methylthioadenosine

Name: 5'-Methylthioadenosine
Brand: TargetMol
SKU: T8227
Price: 39 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T8227Cas No. 2457-80-9

Alias Methylthioadenosine, 5'-S-Methyl-5'-thioadenosine, 5'-Deoxy-5'-(methylthio)adenosine, 5'-(Methylthio)-5'-deoxyadenosine

4-Methyl-2-oxopentanoic acid is an abnormal metabolite, a neurotoxin and a metabolic toxin.

5'-Methylthioadenosine

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Purity: 99.34%

Catalog No. T8227Alias Methylthioadenosine, 5'-S-Methyl-5'-thioadenosine, 5'-Deoxy-5'-(methylthio)adenosine, 5'-(Methylthio)-5'-deoxyadenosineCas No. 2457-80-9

4-Methyl-2-oxopentanoic acid is an abnormal metabolite, a neurotoxin and a metabolic toxin.

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$39	In Stock	In Stock
50 mg	$57	In Stock	In Stock
100 mg	$81	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.34%

Color:White to Yellow

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COA HPLC LCMS HNMR

Product Introduction

5'-Methylthioadenosine AI Summary

5'-Methylthioadenosine exhibits a diverse range of bioactivities and enzyme inhibition properties. It has an affinity constant of 10.0 µM for the A2 receptor and demonstrates significant interaction with various adenosine receptors, exhibiting Ki values of 150-240 nM for A1, 1130-1180 nM for A2A, 13900 nM for A2B, and 680 nM for A3 receptors. It is also effective in inhibiting forskolin-stimulated cAMP accumulation in cells expressing the human adenosine A3 receptor, with an IC50 of 71000 nM. Additionally, 5'-Methylthioadenosine inhibits indole N-methyl-transferase (Ki of 700000 nM) and shows low inhibitory activity against Catechol O-methyltransferase (Ki > 5400000 nM). It also inhibits Escherichia coli cyclopropane fatty acid synthase (Ki = 1500000 nM) and demonstrates inhibitory effects on spermine and spermidine synthase in various tissues. Regarding its anticancer properties, 5'-Methylthioadenosine inhibits the growth of L1210 and L5178Y murine leukemia cells with IC50 values of 600000 nM and 60000 nM, respectively, and shows activity against human cancer cells (IC50 of 150000 nM on CCRF-CEM and 11000 nM on MOLT-4 cells). It affects polyamine metabolism by modulating spermine, spermidine, and putrescine pools and enzyme activities in these cancer cells. Moreover, 5'-Methylthioadenosine has a Km value of 5000 nM for human methylthioadenosine phosphorylase (MTAP) and shows bioactivity related to S-adenosyl-homocysteine deaminase from Thermotoga maritima. 5'-Methylthioadenosine also displays potential as an anti-malarial agent by inhibiting Plasmodium falciparum spermidine synthase (IC50 of 159000 nM) and exhibits antitrypanosomal activity against Trypanosoma brucei brucei with an IC50 of 100000 nM. Finally, it inhibits human PRMT5/MEP50 with a dissociation constant (Kd) of 661 nM, further indicating its multi-faceted bioactivity profile..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	4-Methyl-2-oxopentanoic acid is an abnormal metabolite, a neurotoxin and a metabolic toxin.
Synonyms	Methylthioadenosine, 5'-S-Methyl-5'-thioadenosine, 5'-Deoxy-5'-(methylthio)adenosine, 5'-(Methylthio)-5'-deoxyadenosine

Chemical Properties

Molecular Weight	297.33
Formula	C₁₁H₁₅N₅O₃S
Cas No.	2457-80-9
Smiles	CSC[C@H]1O[C@H]([C@H](O)[C@@H]1O)n1cnc2c(N)ncnc12
Relative Density.	1.85 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMF: 2.5 mg/mL (8.41 mM), Sonication is recommended.

H2O: 1 mg/mL (3.36 mM), Sonication is recommended.

DMSO: 250 mg/mL (840.82 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMF/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3633 mL	16.8163 mL	33.6327 mL	168.1633 mL

DMF/DMSO

	1mg	5mg	10mg	50mg
5 mM	0.6727 mL	3.3633 mL	6.7265 mL	33.6327 mL

DMSO

	1mg	5mg	10mg	50mg
10 mM	0.3363 mL	1.6816 mL	3.3633 mL	16.8163 mL
20 mM	0.1682 mL	0.8408 mL	1.6816 mL	8.4082 mL
50 mM	0.0673 mL	0.3363 mL	0.6727 mL	3.3633 mL
100 mM	0.0336 mL	0.1682 mL	0.3363 mL	1.6816 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc