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Masitinib

Catalog No. T2609   CAS 790299-79-5
Synonyms: AB1010

Masitinib (AB1010) is a tyrosine-kinase inhibitor used in the treatment of mast cell tumors in animals, specifically dogs. Since its introduction in November 2008 it has been distributed under the commercial name Masivet. It has been available in Europe since the second part of 2009. In the USA it is distributed under the name Kinavet and has been available for veterinaries since 2011.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.
Masitinib Chemical Structure
Masitinib, CAS 790299-79-5
Pack Size Availability Price/USD Quantity
5 mg In stock $ 31.00
10 mg In stock $ 48.00
50 mg In stock $ 90.00
100 mg In stock $ 162.00
200 mg In stock $ 252.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 100%
Purity: 99.91%
Purity: 99.55%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Masitinib (AB1010) is a tyrosine-kinase inhibitor used in the treatment of mast cell tumors in animals, specifically dogs. Since its introduction in November 2008 it has been distributed under the commercial name Masivet. It has been available in Europe since the second part of 2009. In the USA it is distributed under the name Kinavet and has been available for veterinaries since 2011.
Targets&IC50 PDGFRβ:800 nM, PDGFRα:540 nM, Kit:200 nM, Abl1:1.20 μM, Lyn B:510 nM
Kinase Assay In vitro enzyme-linked immunoassay with recombinant protein kinases: A 96-well microtitre plateis coated overnight with 0.25?mg/ml poly(Glu,Tyr 4:1), rinsed twice with 250?μL of washing buffer (10 mM phosphate-buffered saline [pH?7.4] and 0.05% Tween?20) and dried for 2 hours at room temperature. Assays are performed at room temperature with a final volume of 50?μL in kinase buffer (10 mM?MgCl2, 1 mM MnCl2, 1 mM sodium orthovanadate, 20 mM HEPES, pH?7.8) containing ATP at a concentration of at least twice the Km for each enzyme and an appropriate amount of recombinant enzyme to ensure a linear reaction rate. Reactions are initiated upon introduction of the enzyme and terminated with the addition of one reaction volume (50 μL) of 100?mM EDTA per 5?M urea mix. Plates are washed three times and incubated with 1:30,000 horseradish peroxidase-conjugated anti-phosphotyrosine monoclonal antibody, then washed three times and incubated with tetramethylbenzidine. The final reaction product is quantified by spectrophotometry at 450?nm.
Cell Research For the assay of Ba/F3 cell proliferation, microtitre plates are seeded with a total of 104 cells/well in 100 μL of RPMI 1640 medium with 10% foetal bovine serum at 37 °C. These are supplemented, or not, with either 0.1% conditioned medium from X63-IL-3 cells or 250 ng/mL murine SCF. The murine SCF, which activates Kit, is purified from the conditioned medium of SCF-producing CHO cells. Cells are grown for 48 hours at 37 °C with Masitinib and then incubated with 10 μL/well of WST-1 reagent for 3 hours at 37 °C. The amount of formazan dye formed is quantified by its absorbance at 450 nm using a scanning multiwell spectrophotometer. A blank well without cells is used as a background control for the spectrophotometer. (Only for Reference)
Synonyms AB1010
Molecular Weight 498.64
Formula C28H30N6OS
CAS No. 790299-79-5

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 93 mg/mL (186.5 mM)

H2O: <1 mg/mL

Ethanol: 4 mg/mL (8.02 mM)

TargetMolReferences and Literature

1. Dubreuil P, et al. PLoS One, 2009, 4(9), e7258. 2. Lawrence J, et al. Vet Comp Oncol, 2011, doi: 10.1111/j.1476-5829.2011.200291.x. 3. Hahn KA, et al. J Vet Intern Med, 2008, 22(6), 1301-1309. 4. Humbert M, et al. PLoS One, 2010, 5(3), e9430.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Cancer Approved Drug Library Anti-Cancer Clinical Compound Library Anti-Cancer Active Compound Library Anti-Cancer Drug Library Tyrosine Kinase Inhibitor Library Immunology/Inflammation Compound Library Osteogenesis Compound Library Orally Active Compound Library Target-Focused Phenotypic Screening Library Anti-Fibrosis Compound Library

Related Products

Related compounds with same targets
Bosutinib D8 SIAIS100 TFA c-ABL-IN-5 BCR-ABL-IN-8 Cenisertib BCR-ABL-IN-7 BCR-ABL-IN-1 SIAIS178

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Keywords

Masitinib 790299-79-5 Angiogenesis Apoptosis Cytoskeletal Signaling Tyrosine Kinase/Adaptors Bcr-Abl FGFR FAK c-Fms c-Kit Src PDGFR Hck PTK2 SCFR inhibit CD117 Focal adhesion kinase PTK2 protein tyrosine kinase 2 AB-1010 AB1010 Inhibitor Platelet-derived growth factor receptor Fibroblast growth factor receptor AB 1010 inhibitor

 

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