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Anti-PDGF R beta/CD140b Polyclonal Antibody
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Catalog No. TMAB-01350
Anti-PDGF R beta/CD140b Polyclonal Antibody is a Rabbit antibody targeting PDGF R beta/CD140b. Anti-PDGF R beta/CD140b Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-PDGF R beta/CD140b Polyclonal Antibody
Anti-PDGF R beta/CD140b Polyclonal Antibody
😃Good
Catalog No. TMAB-01350
Anti-PDGF R beta/CD140b Polyclonal Antibody is a Rabbit antibody targeting PDGF R beta/CD140b. Anti-PDGF R beta/CD140b Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $222 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $527 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Mouse,Dog,Cow) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Cow) |
| Verified Activity | 1. Sample: DU145 (Human) Cell Lysate at 30 μg Primary: Anti-PDGF Receptor beta (TMAB-01350) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kDa Observed band size: 118 kDa 2. Sample: U251 (Human) Cell Lysate at 30 μg Primary: Anti-PDGF Receptor beta (TMAB-01350) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kDa Observed band size: 118 kDa 3. Sample: NIH/3T3 (Rat) Cell Lysate at 30 μg Primary: Anti-PDGF Receptor beta (TMAB-01350) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 118 kDa Observed band size: 118 kDa 4. Tissue/cell: Human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-PDGFRB Polyclonal Antibody, Unconjugated (TMAB-01350) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane; Single-pass type I membrane protein. Cytoplasmic vesicle. Lysosome lumen. Note=After ligand binding, the autophosphorylated receptor is ubiquitinated and internalized, leading to its degradation |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. [provided by RefSeq]. |
| Immunogen | KLH conjugated synthetic peptide: human PDGF-R-B |
| Antigen Species | Human |
| Gene ID | 5159 |
| Uniprot ID | |
| Biology Area | PDGF,Growth factor receptors,Growth Factors,Inflammatory mediators,SARS Coronavirus,PDGF,Receptor Tyrosine Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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