Shopping Cart
- Remove All
Your shopping cart is currently empty
Anti-FAK Polyclonal Antibody
😃Good
Catalog No. TMAB-00652
Alias PTK 2, Protein-tyrosine kinase 2, Protein Tyrosine Kinase Cytoplasmic, Protein tyrosine kinase 2, Protein phosphatase 1 regulatory subunit 71, PPP1R71, pp125FAK, p125FAK, FRNK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1, FAK related non kinase polypeptide, FAK 1, FADK 1, FADK
Anti-FAK Polyclonal Antibody is a Rabbit antibody targeting FAK. Anti-FAK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Anti-FAK Polyclonal Antibody
Anti-FAK Polyclonal Antibody
😃Good
Catalog No. TMAB-00652Alias PTK 2, Protein-tyrosine kinase 2, Protein Tyrosine Kinase Cytoplasmic, Protein tyrosine kinase 2, Protein phosphatase 1 regulatory subunit 71, PPP1R71, pp125FAK, p125FAK, FRNK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1, FAK related non kinase polypeptide, FAK 1, FADK 1, FADK
Anti-FAK Polyclonal Antibody is a Rabbit antibody targeting FAK. Anti-FAK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
Add to Cart
Add to Quotation
Bulk & Custom
Questions
View MoreContact us for more batch information
Resource Download
Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-FAK Polyclonal Antibody is a Rabbit antibody targeting FAK. Anti-FAK Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | PTK 2, Protein-tyrosine kinase 2, Protein Tyrosine Kinase Cytoplasmic, Protein tyrosine kinase 2, Protein phosphatase 1 regulatory subunit 71, PPP1R71, pp125FAK, p125FAK, FRNK, Focal adhesion kinase-related nonkinase, Focal adhesion kinase 1, FAK1, FAK related non kinase polypeptide, FAK 1, FADK 1, FADK |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Tissue/cell: mouse lung tissue;4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-FAK/PTK2 Polyclonal Antibody, Unconjugated (TMAB-00652) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 μg/ml,blue) was used to stain the cell nucleus. 2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00652) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Cerebrum (Mouse) Lysate at 40 μg Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg Lane 3: Cerebrum (Rat) Lysate at 40 μg Lane 4: 293T (Human) Cell Lysate at 30 μg Lane 5: A431 (Human) Cell Lysate at 30 μg Lane 6: Huvec (Human) Cell Lysate at 30 μg Lane 7: A549 (Human) Cell Lysate at 30 μg Primary: Anti-FAK (TMAB-00652) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 125 kDa Observed band size: 120 kDa 4. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00652) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 5. Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00652) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Nucleus. Note=Constituent of focal adhesions. Detected at microtubules. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity. [SUBCELLULAR LOCATION] Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note=Constituent of focal adhesions. |
| Immunogen | KLH conjugated synthetic peptide: human FAK |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID | |
| Biology Area | FAK family,Integrins,Focal Adhesions,FAK / PYK |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Calculator
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Sci Citations
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
Preview
Related Tags: buy Anti-FAK Polyclonal Antibody | purchase Anti-FAK Polyclonal Antibody | Anti-FAK Polyclonal Antibody cost | order Anti-FAK Polyclonal Antibody
- TOP
Feedback
Hello! How can I help you today? 
Copyright © 2015-2025 TargetMol Chemicals Inc. All Rights Reserved.