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FGFR2 beta (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated

(Synonyms: K-sam, KSAM, KGFR, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR2 β (IIIc), FGFR-2, CD332, BEK) Copy Product Info

Synonyms: K-sam, KSAM, KGFR, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR2 β (IIIc), FGFR-2, CD332, BEK

Catalog No. TMPK-00397 Copy Product Info
FGFR2 beta (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P21802-1.

Batch Information

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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Immobilized Human FGF basic, No Tag at 5 μg/mL (100 μL/well) on the plate. Dose response curve for Biotinylated Human FGFR2 beta (IIIc), His Tag with the EC50 of 1.25 μg/mL determined by ELISA.
Description
FGFR2 beta (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 28.2 kDa and the accession number is P21802-1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberP21802-1
ConstructionArg152-Glu377
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsK-sam, KSAM, KGFR, Keratinocyte growth factor receptor, Fibroblast growth factor receptor 2, FGFR2 β (IIIc), FGFR-2, CD332, BEK
Research Background
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the alpha  isoform, or only IgII and IgIII, referred to as the beta  isoform.
Chemical Properties
Molecular Weight28.2 kDa (predicted). Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

Related Tags: FGFR2 beta (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | FGFR2 beta (IIIc) Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight