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c-Kit/CD117 Protein, Human, Recombinant (His & Avi), Biotinylated
(Synonyms: SCFR, PBT, KIT, EC 2.7.10.1, EC 2.7.10, c-kit, ckit, CD117) Copy Product InfoSynonyms: SCFR, PBT, KIT, EC 2.7.10.1, EC 2.7.10, c-kit, ckit, CD117
Catalog No. TMPK-00805 Copy Product Info
The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 58.60 kDa and the accession number is P10721-2.
| Pack Size | Price | USA Stock | Global Stock | Quantity |
|---|---|---|---|---|
| 5 µg | $91 | - | In Stock | |
| 10 µg | $148 | - | In Stock | |
| 20 µg | $245 | - | In Stock | |
| 50 µg | $483 | - | In Stock | |
| 100 µg | $813 | - | In Stock | |
| 200 µg | $1,470 | 7-10 days | 7-10 days | |
| 500 µg | $3,250 | 7-10 days | 7-10 days |
For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
Appearance:Lyophilized powder
Color:White
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Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Bioactivity | Immobilized Biotinylated Human CD117, His-Avi Tag at 1 μg/ml (100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-CD117 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA (QC Test). Immobilized Human SCF, No Tag at 5 μg/ml (100 μl/well) on the plate. Dose response curve for Biotinylated Human CD117, His Tag with the EC50 of 6.8 μg/ml determined by ELISA. |
| Description | The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. CD117 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 58.60 kDa and the accession number is P10721-2. |
| Species | Human |
| Expression System | HEK293 Cells |
| Tag | C-His-Avi |
| Accession Number | P10721-2 |
| Construction | Gln26-Pro520 |
| Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
| Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
| Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| Synonyms | SCFR, PBT, KIT, EC 2.7.10.1, EC 2.7.10, c-kit, ckit, CD117 |
| Research Background | The c-kit proto-oncogen (CD 117) has been shown to be present in several cell types including normal and neoplastic hemopoietic cells. Among normal BM cells, CD117 expression has been found in about half of the CD34 precursors including progenitors committed to the erythroid, granulo-monocytic, and megakaryocytic cell lineages. |
| Molecular Weight | 58.60 kDa (predicted). Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result. |
| Shipping | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
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Related Tags: c-Kit/CD117 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | c-Kit/CD117 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight
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