Antibody Type: Rabbit Polyclonal

Application: WB,IHC-P,IHC-Fr,IF

Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

IgG

Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)

1. Sample:
Lane 1: Testis (Mouse) Lysate at 40 μg
Lane 2: NIH/3T3 (Mouse) Cell Lysate at 30 μg
Lane 3: Cerebrum (Mouse) Lysate at 40 μg
Lane 4: Testis (Mouse) Lysate at 40 μg
Lane 5: 293T (Human) Cell Lysate at 30 μg
Lane 6: A431 (Human) Cell Lysate at 30 μg
Lane 7: Huvec (Human) Cell Lysate at 30 μg
Lane 8: MDA-MB-231 (Human) Cell Lysate at 30 μg
Lane 9: A549 (Human) Cell Lysate at 30 μg
Primary: Anti-FAK (TMAB-00653) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 125 kDa
2. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00653) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00653) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
4. Paraformaldehyde-fixed, paraffin embedded (mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00653) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
5. Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00653) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
6. Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00653) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
7. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (FAK) Polyclonal Antibody, Unconjugated (TMAB-00653) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
8. Sample:
MCF-7 (Human) Cell Lysate at 30 μg
LympHnode (Mouse) Lysate at 40 μg
Primary: Anti-FAK (TMAB-00653) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 116 kDa

WB,IHC-P,IHC-Fr,IF

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human FAK

Human

5747

FAK family,Integrins,Focal Adhesions,FAK / PYK