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Emodin

Catalog No. T2869   CAS 518-82-1
Synonyms: Frangula emodin

Emodin (Frangula emodin) is a naturally occurring anthraquinone present in the roots and barks of numerous plants; exerts antiproliferative effects in Y cells that are regulated by different signaling pathways.

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Emodin Chemical Structure
Emodin, CAS 518-82-1
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50 mg In stock $ 45.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 98.9%
Purity: 94.4%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Emodin (Frangula emodin) is a naturally occurring anthraquinone present in the roots and barks of numerous plants; exerts antiproliferative effects in Y cells that are regulated by different signaling pathways.
Targets&IC50 CKII:2 μM.
In vitro Emodin, an anthraquinone derivative, selectively inhibits casein kinase II(CKII), a Ser/Thr kinase, as a competitive inhibitor. Emodin inhibits CKII activity with IC50 of 2 μM, which is two to three orders of magnitude lower than those against the other kinases. Enzyme kinetic assays show that Emodin inhibits CKII activity as acompetitive inhibitor against ATP with Ki of 7.2 μM[1]. Emodin is a broad-spectrum inhibitory agent of cancer cells, including leukemia, lung cancer, human tongue squamous cancer, colon cancer, gallbladder cancer, pancreatic cancer, breast cancer, human cervical cancer and hepatic carcinoma cells. Emodin inhibits A549, HepG2, OVCAR-3, HeLa and Madin-Darby Canine Kidney (MDCK) cells with IC50 of 19.54, 12.79, 25.82, 12.14 and 5.81 μg/mL. The anticancer mechanisms of Emodin are involved in many biological pathways, such as casein kinase Ⅱ and ERK1/2[2]. Emodin is applied as a Reactive oxygen species (ROS) generator in combination with cisplatin in T24 and J82 human bladder cancer cells. Emodin kills T24 and J82 cells in the dose-dependent and time-dependent manner, and it is less toxic to HCV-29 cells. The concentration of 20 and 15 μM is selected as appropriate doses for investigating chemotherapeutic sensitivity of T24 and J82 cells at 24 h, respectively[3].
In vivo Mice treated with Emodin (50 mg/kg) and Cisplatin (1 mg/kg) have significantly smaller tumors than those from the other groups. In addition, no notable differences on the body weight loss are observed among groups and no obvious necrosis and abnormity are observed in the sections of liver, kidney and heart. These results demonstrate that Emodin/cisplatin co-treatment can significantly suppress tumor growth in vivo with no distinct side effects. Consistent with in vitro experiment, TUNEL assay shows that Emodin/cisplatin combination significantly increased cell apoptosis in xenograft tumors. Emodin/Cisplatin co-treatment group also has lower MRP1 expression than the other groups[3].
Cell Research Emodin is prepared in DMSO and stored, and then diluted with appropriate medium before use[3]. The T24 human bladder cancer cells, the HCV-29 normal bladder epithelial cells and J82 human bladder cancer cells are are cultured in RPMI 1640 medium supplemented with 10 % fetal bovine serum at 37°C in a humidified atmosphere containing 5 % CO2. Cells are seeded in 96-well plates with 2×104 cells per well. The cells are incubated with Emodin for 24 h at different concentrations (0, 5, 10, 20, 30, 40, 50, 60, 70 μM) and chose the critical concentration (20 μM) treated with cells for 0, 6, 12, 24, 48, 72, 96 h. The cells are incubated with cisplatin for 24 h at different concentrations (0, 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 μg/mL). MTT assay is used to analyze the cell viability. Cells are treated with drugs for 24 h and apoptotic rates are assessed with flow cytometry using AnnexinV-fluorescein isothiocyanate (AnnexinV-FITC)/propidium iodide (PI) kit. Samples are prepared according to the manufacturer's instruction and analyzed by a flow cytometry (FCM) Calibur[3].
Source
Synonyms Frangula emodin
Molecular Weight 270.24
Formula C15H10O5
CAS No. 518-82-1

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 54 mg/mL (199.82 mM)

H2O: <1 mg/mL

Ethanol: 3 mg/mL (11.1 mM)

TargetMolReferences and Literature

1. Yim H, et al. Emodin, an anthraquinone derivative isolated from the rhizomes of Rheum palmatum, selectively inhibits the activity of casein kinase II as a competitive inhibitor. Planta Med. 1999 Feb;65(1):9-13. 2. Xing JY, et al. Antitumor Effects and Mechanism of Novel Emodin Rhamnoside Derivatives against Human Cancer CellsIn Vitro. PLoS One. 2015 Dec 18;10(12):e20144781. 3. Li X, et al. Emodin enhances cisplatin-induced cytotoxicity in human bladder cancer cells through ROS elevation and MRP1 downregulation. BMC Cancer. 2016 Aug 2;16:578. 5. Costa M, Rosa F, Ribeiro T, et al. Identification of Cyanobacterial Strains with Potential for the Treatment of Obesity-Related Co-Morbidities by Bioactivity, Toxicity Evaluation and Metabolite Profiling[J]. Marine drugs. 2019 May 10;17(5).

TargetMolCitations

1. Li C, Zhou H, Guo L, et al. Potential inhibitors for blocking the interaction of the coronavirus SARSCoV-2 spike protein and its host cell receptor ACE2. Journal of Translational Medicine. 2022, 20(1): 1-11. 2. Costa M, Rosa F, Ribeiro T, et al. Identification of Cyanobacterial Strains with Potential for the Treatment of Obesity-Related Co-Morbidities by Bioactivity, Toxicity Evaluation and Metabolite Profiling. Marine Drugs. 2019 May 10;17(5) 3. Yang N, Li C, Li H, et al. Emodin Induced SREBP1-Dependent and SREBP1-Independent Apoptosis in Hepatocellular Carcinoma Cells. Frontiers in pharmacology. 2019, 10. 4. Zhang H, Cai J, Li C, et al.Wogonin inhibits latent HIV-1 reactivation by downregulating histone crotonylation.Phytomedicine.2023: 154855.

Related compound libraries

This product is contained In the following compound libraries:
Miao medicine Compound Library Anti-Cancer Active Compound Library Anti-Infection Compound Library Natural Product Library for HTS Anti-Aging Compound Library Food as Medicine Compound Library Bioactive Lipid Compound Library Kinase Inhibitor Library Traditional Chinese Medicine Monomer Library Wnt/Hedgehog/Notch Compound Library

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Keywords

Emodin 518-82-1 Autophagy Metabolism Microbiology/Virology Stem Cells SARS-CoV Casein Kinase CK2 inhibit metabolic kinase Inhibitor 11β-HSD1 Spike Frangula emodin SARS casein mice insulin diabete resistance syndrome protein SARS coronavirus coronavirus inhibitor

 

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