Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC). The Specific Activity is >80pmols/min/μg

SARS-CoV-2 Plpro/papain-like protease Protein (aa 1564-1880, His) is expressed in E. coli expression system. The predicted molecular weight is 36.79 kDa and the accession number is QVD65746.1.

SARS-CoV-2

E. coli

A DNA sequence encoding the SARS-CoV-2 (2019-nCoV) papain-like protease (QVD65746.1) (Glu1564-Val1880) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: Met

> 90 % as determined by SDS-PAGE.

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The coronaviral proteases, papain-like protease (PLpro) and 3C-like protease (3CLpro), are attractive antiviral drug targets because they are essential for coronaviral replication. PLpro has the additional function of stripping ubiquitin and ISG15 from host-cell proteins to aid coronaviruses in their evasion of the host innate immune responses. Targeting PLpro with antiviral drugs may have an advantage in not only inhibiting viral replication but also inhibiting the dysregulation of signaling cascades in infected cells that may lead to cell death in surrounding, uninfected cells.