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CCCP

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Purity:99.64%

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COA HNMR LCMS

CCCP

Catalog No. T7081Cas No. 555-60-2

CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. CCCP inhibits the activation of STING and its downstream signaling molecules TBK1 and IRF3.

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Pack Size	Price	Availability
100 mg	$41	In Stock
200 mg	$58	In Stock
500 mg	$97	In Stock
1 mL x 10 mM (in DMSO)	$45	In Stock

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Product Introduction

Bioactivity

Description	CCCP (Carbonyl Cyanide m-Chlorophenylhydrazone) is an oxidative phosphorylation (OXPHOS) inhibitor and mitochondrial proton carrier uncoupler. CCCP inhibits the activation of STING and its downstream signaling molecules TBK1 and IRF3.
In vitro	METHODS: Human cervical cancer cells HeLa were treated with CCCP (20 μM) for 30 min, and the effect on the mitochondria-labeled fluorescent dye DiOC6 was observed using live cell fluorescence microscopy. RESULTS: CCCP-induced loss of mitochondrial membrane potential resulted in efflux of DiOC6 from mitochondria into the cytoplasm and subsequent localization to punctate structures. [1] METHODS: Mouse alveolar epithelial cells, MLE-12, were treated with CCCP (10-50 μM) for 2-18 h. The expression levels of the target proteins were examined by Western Blot. RESULTS: CCCP caused a dose- and time-dependent conversion of LC3-I to LC3-II. [2]
In vivo	METHODS: To assay in vivo activity, CCCP (5 mg/kg) was administered as a single intraperitoneal injection to adult and aged C57BL/6J mice, and hearts were collected 12 h later. RESULTS: Under CCCP-treated conditions, more mitochondria bound to autophagic vesicles were found in the hearts of adult but not aged mice. [3] METHODS: To assay in vivo activity, CCCP (1 mg/kg) was administered intraperitoneally to mt-Rosella mice once a day for three days. RESULTS: Mitochondrial fragments were readily visible on the hearts of CCCP-treated mice, and these fragments were trapped in lysosomes for degradation.CCCP induced mitochondrial autophagy. [4]
Alias	Carbonyl Cyanide m-Chlorophenylhydrazone, Carbonyl cyanide 3-chlorophenylhydrazone

Chemical Properties

Molecular Weight	204.62
Formula	C9H5ClN4
Cas No.	555-60-2

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 60 mg/mL (293.23 mM)

Ethanol: 5 mg/mL

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	4.8871 mL	24.4355 mL	48.8711 mL	244.3554 mL
5 mM	0.9774 mL	4.8871 mL	9.7742 mL	48.8711 mL
10 mM	0.4887 mL	2.4436 mL	4.8871 mL	24.4355 mL
20 mM	0.2444 mL	1.2218 mL	2.4436 mL	12.2178 mL
50 mM	0.0977 mL	0.4887 mL	0.9774 mL	4.8871 mL
100 mM	0.0489 mL	0.2444 mL	0.4887 mL	2.4436 mL

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