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5-Fluorouridine

Name: 5-Fluorouridine
Brand: TargetMol
SKU: T1349
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1349 Copy Product Info

Purity: 99.85%

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5-Fluorouridine is a metabolite of 5-fluorouracil that inhibits rRNA synthesis in human colon cancer cells and exhibits antitumor and antiviral activity. 5-Fluorouridine exerts a cytotoxic effect on the growth of L1210 cells, with an IC₅₀ value of 2 nM. 5-Fluorouridine binds to poly-A RNA and exhibits antiproliferative activity. 5-Fluorouridine can be used in studies of nucleic acid metabolism, cancer chemotherapy, and viral replication mechanisms.

Cas No. 316-46-1

Pack Size	Price	USA Stock	Global Stock
100 mg	$39	In Stock	In Stock
200 mg	$55	In Stock	In Stock
500 mg	$91	-	In Stock
1 mL x 10 mM (in DMSO)	$30	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

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For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:99.85%

Appearance:Solid

Color:White

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COA LCMS HNMR

Product Introduction

5-Fluorouridine AI Summary

5-Fluorouridine exhibits a broad spectrum of potent bioactivities, particularly related to its antitumor properties. It shows strong cytotoxic effects against various cancer cell lines, including L1210 mouse leukemia cells (ID50 of 0.0024 uM) and other cell lines like MOLT-3, U-937, IM-9, and K-562, with IC50 values typically below 0.01 μg/mL. The compound specifically demonstrates powerful inhibitory activities against stomach and colon tumor cell lines, with IC50 values ranging from 5.4nM to 73.0nM. In in vivo models, 5-Fluorouridine prolongs the survival of mice inoculated with leukemia cells and shows dose-dependent effects on weight change and lifespan. Additionally, 5-Fluorouridine inhibits the growth of Mycobacterium tuberculosis by targeting thymidine monophosphate kinase (TMPK), with a Ki value of 521,000 nM, and exhibits competitive inhibition against bovine pancreatic RNase A, with a Ki value of 33600.0 nM. It also displays cytostatic activity in mouse and human leukemia cells, including those deficient in deoxythymidine kinase, and shows altered activity in the presence of different nucleosides and nucleobases, particularly uracil. Further evaluations reveal that 5-Fluorouridine has significant inhibitory effects against various cell lines, including leukemia, renal, lung, colon, melanoma, and ovarian, and has demonstrated efficacy in inhibiting viral activities against Hepatitis C, Lassa Virus, and Marburg Virus. It also inhibits the Menin-MLL interaction, hemoglobin beta chain splicing, and DNA re-replication, highlighting its potential in cancer therapeutic applications and beyond..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description

In vivo

Methods: Normal ddY mice were immunized with sheep red blood cells (SRBC) via intravenous injection. From the day of immunization, 5-Fluorouridine (0.025, 0.05, 0.1 mmol/kg) was administered orally once daily for 10 consecutive days. Blood samples were collected on days 5 and 10 post-immunization, and IgM and IgG antibody titers against SRBC were detected by ELISA.
Results: At a dose of 0.1 mmol/kg, 5-Fluorouridine significantly suppressed the production of both IgM and IgG antibodies.[1]

Chemical Properties

Molecular Weight	262.19
Formula	C₉H₁₁FN₂O₆
Cas No.	316-46-1
Smiles	O[C@H]1[C@@H](O[C@H](CO)[C@H]1O)N2C(=O)NC(=O)C(F)=C2
Relative Density.	1.77g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (953.51 mM), Sonication is recommended.

In Vivo Formulation

5% DMSO+95% Saline: 1.25 mg/mL (4.77 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.8140 mL	19.0701 mL	38.1403 mL	190.7014 mL
5 mM	0.7628 mL	3.8140 mL	7.6281 mL	38.1403 mL
10 mM	0.3814 mL	1.9070 mL	3.8140 mL	19.0701 mL
20 mM	0.1907 mL	0.9535 mL	1.9070 mL	9.5351 mL
50 mM	0.0763 mL	0.3814 mL	0.7628 mL	3.8140 mL
100 mM	0.0381 mL	0.1907 mL	0.3814 mL	1.9070 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.