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1400W dihydrochloride

Name: 1400W dihydrochloride
Brand: TargetMol
SKU: T3491
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3491Cas No. 214358-33-5

Alias N-(3-(Aminomethyl)benzyl)acetamidine, 1400W 2HCl

1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).

1400W dihydrochloride

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Purity: 99.94%

Catalog No. T3491Alias N-(3-(Aminomethyl)benzyl)acetamidine, 1400W 2HClCas No. 214358-33-5

1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).

Pack Size	Price	Availability
5 mg	$43	In Stock
10 mg	$68	In Stock
25 mg	$127	In Stock
50 mg	$231	In Stock
100 mg	$393	In Stock
200 mg	$511	In Stock
500 mg	$829	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock

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Purity:99.94%

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).
Targets&IC50	eNOS:50 μM(Ki), nNOS:2 μM(Ki), iNOS:<7 nM(Kd)
In vitro	1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1].
In vivo	1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4].
Cell Research	RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody. (Only for Reference)
Synonyms	N-(3-(Aminomethyl)benzyl)acetamidine, 1400W 2HCl

Chemical Properties

Molecular Weight	250.17
Formula	C₁₀H₁₇Cl₂N₃
Cas No.	214358-33-5
Smiles	Cl.Cl.CC(=N)NCc1cccc(CN)c1
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 139.9 mM, Sonication is recommended.

DMSO: 55 mg/mL (219.85 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.99 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9973 mL	19.9864 mL	39.9728 mL	199.8641 mL
5 mM	0.7995 mL	3.9973 mL	7.9946 mL	39.9728 mL
10 mM	0.3997 mL	1.9986 mL	3.9973 mL	19.9864 mL
20 mM	0.1999 mL	0.9993 mL	1.9986 mL	9.9932 mL
50 mM	0.0799 mL	0.3997 mL	0.7995 mL	3.9973 mL
100 mM	0.0400 mL	0.1999 mL	0.3997 mL	1.9986 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc