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Anti-eNOS Antibody (6A162)
😃Good
Catalog No. TMAB-00615
Anti-eNOS Antibody (6A162) is a Mouse antibody targeting eNOS. Anti-eNOS Antibody (6A162) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-eNOS Antibody (6A162)
Anti-eNOS Antibody (6A162)
😃Good
Catalog No. TMAB-00615
Anti-eNOS Antibody (6A162) is a Mouse antibody targeting eNOS. Anti-eNOS Antibody (6A162) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $373 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG |
| Clone | 6A162 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Sample: Liver (mouse) Lysate at 40 μg Primary: Anti-eNOS (TMAB-00615) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 133 kDa Observed band size: 135 kDa 2. Sample: Cerebrum (Mouse) Lysate at 40 μg Cerebrum (Rat) Lysate at 40 μg Lung (Mouse) Lysate at 40 μg Placenta (Mouse) Lysate at 40 μg Primary: Anti-eNOS (TMAB-00615) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 135 kDa Observed band size: 135 kDa 3. Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eNOS/NOS-3) Monoclonal Antibody, Unconjugated (TMAB-00615) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| Immunogen | KLH conjugated synthetic peptide: human eNos |
| Antigen Species | Human |
| Gene ID | 4846 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Platelets,Nitric oxide associated,Oxidative Stress,Hypoxia,Oxidative stress,NOS |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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