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Anti-eNOS Polyclonal Antibody 2
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Catalog No. TMAB-00617
Anti-eNOS Polyclonal Antibody 2 is a Rabbit antibody targeting eNOS. Anti-eNOS Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-eNOS Polyclonal Antibody 2
Anti-eNOS Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00617
Anti-eNOS Polyclonal Antibody 2 is a Rabbit antibody targeting eNOS. Anti-eNOS Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,GuineaPig) |
| Ig Type | IgG |
| Reactivity | Human,Rat (predicted:Mouse,Dog,Pig,Cow,Rabbit,GuineaPig) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (eNOS) Polyclonal Antibody, Unconjugated (TMAB-00617) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining. 2. Sample: K562 (Human) Cell Lysate at 40 μg Primary: Anti-eNOS (TMAB-00617) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kDa Observed band size: 133 kDa 3. Sample: A431 Cell (Human) Lysate at 30 μg Primary: Anti-eNOS (TMAB-00617) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kDa Observed band size: 133 kDa 4. Sample: K562Cell (Human) Lysate at 30 μg Primary: Anti-eNOS (TMAB-00617) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 133 kDa Observed band size: 133 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). |
| Immunogen | KLH conjugated synthetic peptide: human eNos |
| Antigen Species | Human |
| Gene ID | 4846 |
| Uniprot ID | |
| Biology Area | Response to hypoxia,Platelets,Nitric oxide associated,Oxidative Stress,Hypoxia,Oxidative stress,NOS |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
Sci Citations
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
Tech Support
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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