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Anti-eNOS Polyclonal Antibody

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Catalog No. TMAB-00616

Anti-eNOS Polyclonal Antibody is a Rabbit antibody targeting eNOS. Anti-eNOS Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

Anti-eNOS Polyclonal Antibody

Anti-eNOS Polyclonal Antibody

😃Good
Catalog No. TMAB-00616
Anti-eNOS Polyclonal Antibody is a Rabbit antibody targeting eNOS. Anti-eNOS Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.
Pack SizePriceAvailabilityQuantity
50 μL$221 7-10 days
100 μL$373 7-10 days
200 μL$529 7-10 days
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Product Introduction

Bioactivity
Description
Antibody Type: Rabbit Polyclonal

Application: IHC-P,IHC-Fr,IF

Reactivity: Human,Rat (predicted:Mouse,Dog,Pig,Cow,Sheep,GuineaPig)
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse,Dog,Pig,Cow,Sheep,GuineaPig)
Verified Activity
Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-NOS-3/eNOS Polyclonal Antibody, Unconjugated (TMAB-00616) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
Application
IHC-P,IHC-Fr,IF
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundNitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS).
Antigen Details
Immunogen
KLH conjugated synthetic peptide: mouse eNOS
Antigen Species
Mouse
Gene ID
18127
Uniprot ID
Biology Area
Response to hypoxia,Platelets,Nitric oxide associated,Oxidative Stress,Hypoxia,Oxidative stress,NOS
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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