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Nisoldipine

Name: Nisoldipine
Brand: TargetMol
SKU: T0163
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0163Cas No. 63675-72-9

Alias BAY-k 5552

Nisoldipine (BAY-k 5552) is a dihydropyridine calcium channel antagonist that acts as a potent arterial vasodilator and antihypertensive agent.

Nisoldipine

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Purity: 99.57%

Catalog No. T0163Alias BAY-k 5552Cas No. 63675-72-9

Nisoldipine (BAY-k 5552) is a dihydropyridine calcium channel antagonist that acts as a potent arterial vasodilator and antihypertensive agent.

Pack Size	Price	USA Warehouse	Global Warehouse
200 mg	$34	In Stock	In Stock
500 mg	$44	In Stock	In Stock
1 g	$70	In Stock	In Stock
5 g	$168	-	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Purity:99.57%

Appearance:Solid

Color:White to Yellow

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COA HNMR

Product Introduction

Bioactivity

Description	Nisoldipine (BAY-k 5552) is a dihydropyridine calcium channel antagonist that acts as a potent arterial vasodilator and antihypertensive agent.
Targets&IC50	Cav 1.2, L-type:10 nM
In vitro	In patients with Timothy syndrome, Nisoldipine (IC50=267 nM) induces vasodilation and lowers blood pressure by inhibiting the influx of calcium ions through L-type calcium channels, thereby decreasing the contractility of arterial smooth muscle and subsequent vasoconstriction.
In vivo	In guinea pig ventricular myocytes, Nisoldipine acts on delayed rectifier K+ channels, inhibiting the rapid-activating component (IKr) with an IC50 of 23 μM, and the slow-activating component (IKs) with an IC50 of 40 μM.
Kinase Assay	Binding experiments of electrophysiology: CHO cells expressing the subunit of the voltage-dependent L-type Ca2+ channel are cultrured in medium without serum in the presence of different concentrations of Nisoldipine. Then Ca2+ channel current elicited from a holding potential of -100 mV or -50 mV is recorded at room temperature with the whole-cell configuration of the patch-clamp method using the List EPC-7 patch-clamp amplifer and pClamp software. The concentration of competitor inhibiting 50% of the specific binding represents IC50.
Cell Research	The myocytes are bathed in normal Tyrode's solution, held at -80 mV, and depolarised after 200-ms prepulses (-40mV) to more positive potentials for 500 ms at 0.1 Hz, tail currents are recorded on repolarisations to -40mV. The myocytes are exposed to 10-100 mM Nisoldipine for 8-10 minutes. Then the whole-cell membrane currents are recorded using an EPC-7 amplifier.(Only for Reference)
Synonyms	BAY-k 5552

Chemical Properties

Molecular Weight	388.41
Formula	C₂₀H₂₄N₂O₆
Cas No.	63675-72-9
Smiles	C(OCC(C)C)(=O)C=1C(C(C(OC)=O)=C(C)NC1C)C2=C(N(=O)=O)C=CC=C2
Relative Density.	1.205 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 56 mg/mL (144.18 mM), Sonication is recommended.

H2O: < 1 mg/mL (insoluble or slightly soluble)

DMSO: 163 mg/mL (419.66 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (5.15 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.5746 mL	12.8730 mL	25.7460 mL	128.7300 mL
5 mM	0.5149 mL	2.5746 mL	5.1492 mL	25.7460 mL
10 mM	0.2575 mL	1.2873 mL	2.5746 mL	12.8730 mL
20 mM	0.1287 mL	0.6436 mL	1.2873 mL	6.4365 mL
50 mM	0.0515 mL	0.2575 mL	0.5149 mL	2.5746 mL
100 mM	0.0257 mL	0.1287 mL	0.2575 mL	1.2873 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc