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Ethacrynic acid

Name: Ethacrynic acid
Brand: TargetMol
SKU: T4564
Price: 31 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T4564Cas No. 58-54-8

Alias Hydromedin, Etacrynic Acid, Edecrin

Ethacrynic acid (Edecrin) is a nonsulfonamide loop diuretic that inhibits the activity of the Na+/K+/2Cl- cotransporter NKCC2 in the thick ascending limb of the loop of Henle. It also inhibits glutathione S-transferase and Wnt signalling, producing cytotoxicity in chronic lymphocytic leukaemia cells and other tumour cells.

Ethacrynic acid

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Purity: 99.85%

Catalog No. T4564Alias Hydromedin, Etacrynic Acid, EdecrinCas No. 58-54-8

Pack Size	Price	USA Warehouse	Global Warehouse
25 mg	$31	In Stock	In Stock
50 mg	$44	In Stock	In Stock
100 mg	$64	In Stock	In Stock
500 mg	$148	-	In Stock
1 mL x 10 mM (in DMSO)	$32	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

This molecule is a custom-made product. TargetMol has an excellent synthesis team with the experience and capability to provide you with cost-effective products.If you have any questions, please feel free to contact us. We are committed to serving you wholeheartedly.

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.85%

Color:White

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COA LCMS HNMR

Product Introduction

Ethacrynic acid AI Summary

Ethacrynic acid exhibits a diverse range of bioactivities and pharmacological properties. It demonstrates significant diuretic activity in both saline-loaded mice and dogs, leading to increased sodium, potassium, and chloride excretion, as well as elevated urine volume output at various doses. It has a logD7.4 value of 0.02 and a dissociation constant (pKa) around 3.5, suggesting its acid-base behavior and lipophilicity, which impact its absorption and distribution. Additionally, Ethacrynic acid shows antiviral activity against SARS-CoV-2 by inhibiting virus-induced cytotoxicity in Vero E6 and Caco-2 cells, although with moderate potency (IC50 values generally higher than 20,000 nM). It exhibits antimicrobial activity against several pathogens including MRSA, with variable inhibition percentages. The compound also shows cytotoxicity against various cancer cell lines, indicating potential anti-proliferative effects, and induces apoptosis in human HeLa cells. On the enzyme inhibition front, Ethacrynic acid inhibits GST P1-1 with an IC50 of 3400 nM and GSTO1-1 with an IC50 of 25,000 nM. It also inhibits human MRP2 efficiently (IC50 = 3850 nM) and demonstrates inhibition of aldose reductase enzyme (IC50 = 1150 nM). The compound shows moderate to high hepatotoxicity, with evidence of drug-induced liver injury (DILI) and acute liver toxicity in clinical evaluations. Finally, Ethacrynic acid displayed selective inhibition effects on various human cellular pathways and proteins, including MEK6 (IC50 = 4500 nM) and HDAC6, and moderate inhibition of human PHPT1 activity (IC50 = 18,300 nM). Overall, Ethacrynic acid presents a broad spectrum of bioactivities, making it a compound of interest for further pharmacological and toxicological studies..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Ethacrynic acid (Edecrin) is a nonsulfonamide loop diuretic that inhibits the activity of the Na+/K+/2Cl- cotransporter NKCC2 in the thick ascending limb of the loop of Henle. It also inhibits glutathione S-transferase and Wnt signalling, producing cytotoxicity in chronic lymphocytic leukaemia cells and other tumour cells.
Synonyms	Hydromedin, Etacrynic Acid, Edecrin

Chemical Properties

Molecular Weight	303.14
Formula	C₁₃H₁₂Cl₂O₄
Cas No.	58-54-8
Smiles	CCC(=C)C(=O)C1=CC=C(OCC(O)=O)C(Cl)=C1Cl
Relative Density.	1.35g/cm3

Storage & Solubility Information

Storage

keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

Ethanol: 30 mg/mL (98.96 mM), Sonication is recommended.

DMSO: 245 mg/mL (808.21 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (6.6 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.2988 mL	16.4940 mL	32.9881 mL	164.9403 mL
5 mM	0.6598 mL	3.2988 mL	6.5976 mL	32.9881 mL
10 mM	0.3299 mL	1.6494 mL	3.2988 mL	16.4940 mL
20 mM	0.1649 mL	0.8247 mL	1.6494 mL	8.2470 mL
50 mM	0.0660 mL	0.3299 mL	0.6598 mL	3.2988 mL

DMSO

	1mg	5mg	10mg	50mg
100 mM	0.0330 mL	0.1649 mL	0.3299 mL	1.6494 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc