Shopping Cart

Your shopping cart is currently empty

Aplidine

Name: Aplidine
Brand: TargetMol
SKU: T9715
Price: 247 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: Plitidepsin) Copy Product Info

🥰Excellent

Synonyms: Plitidepsin

Catalog No. T9715 Copy Product Info

Purity: 99.86%

🥰Excellent

Aplidine possesses antiviral activity against SARS-CoV-2(IC90 = 0.88 nM). Aplidine is a potent anti-cancer agent by targeting eukaryotic translation elongation factor 1 Alpha 2(EEF1A2, Kd = 80 nM).

Cas No. 137219-37-5

Customer service consultation

Pack Size	Price	USA Stock	Global Stock
1 mg	$247	In Stock	In Stock
5 mg	$619	In Stock	In Stock
10 mg	$828	In Stock	In Stock
25 mg	$1,280	In Stock	In Stock
50 mg	$1,650	In Stock	In Stock
100 mg	$2,660	-	In Stock
1 mL x 10 mM (in DMSO)	$987	In Stock	In Stock

For In stock only · Estimated delivery: USA Stock (1-2 days) Global Stock (5-7 days)

Add to Cart

Add to Quotation

For research use only—not for human use. No sales to individuals. Use as intended only.

Questions

Batch Information

Select Batch

Purity:99.86%

Appearance:Solid

Color:Yellow

Resource Download

COA MS LCMS

Product Introduction

Bioactivity

Description	Aplidine possesses antiviral activity against SARS-CoV-2(IC90 = 0.88 nM). Aplidine is a potent anti-cancer agent by targeting eukaryotic translation elongation factor 1 Alpha 2(EEF1A2, Kd = 80 nM).
Targets & IC50	SARS-CoV-2:0.88 nM(IC90), eEF1A2:80 nM(Kd)
In vitro	In hACE2-293T cells, Aplidine exhibits anti–SARS-CoV-2 activity with an IC90 of 0.88 nM. In an established model of human pneumocyte-like cells, Aplidine inhibits SARS-CoV-2 replication with an IC90 of 3.14 nM and a selectivity index of 40.4[1]. Aplidine (20 nM; 1 h) induces a dose-dependent decrease in VEGF secretion in MOLT-4 cells. Aplidine (20 nM; 1 h) does not result in significant inhibition of VEGF-R1 mRNA in normal endothelial cells, which do express VEGFR-1 but do not secrete VEGF[1].
In vivo	In BALB/c mice expressing human ACE2, Aplidine significantly reduced SARS-CoV-2 infection. 0.3 mg/kg Aplidine group results in a reduction of nearly 2 log units in SARS-CoV-2 viral titers in the lungs, and the 1 mg/kg group leads to a reduction of 1.5 log units[2].
Synonyms	Plitidepsin

Chemical Properties

Molecular Weight	1110.34
Formula	C₅₇H₈₇N₇O₁₅
Cas No.	137219-37-5
Smiles	[H][C@@]12CCCN1C(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)C(=O)[C@@H](OC(=O)C[C@H](O)[C@]([H])(NC(=O)[C@@H](NC(=O)[C@@H](CC(C)C)N(C)C(=O)[C@@H]1CCCN1C(=O)C(C)=O)[C@@H](C)OC(=O)[C@H](Cc1ccc(OC)cc1)N(C)C2=O)[C@@H](C)CC)C(C)C
Relative Density.	1.24 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 50 mg/mL (45.03 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+90% Corn Oil: 3.3 mg/mL (2.97 mM), Sonication is recommended.

10% DMSO+90% (20% SBE-β-CD in Saline): < 5 mg/mL (4.5 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% Saline: < 5 mg/mL (4.5 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: < 5 mg/mL (4.5 mM), Lower concentrations may be soluble, but exact solubility limit is unknown.

10% DMSO+90% Corn oil: 5 mg/mL (4.5 mM), Solution.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	0.9006 mL	4.5031 mL	9.0063 mL	45.0313 mL
5 mM	0.1801 mL	0.9006 mL	1.8013 mL	9.0063 mL
10 mM	0.0901 mL	0.4503 mL	0.9006 mL	4.5031 mL
20 mM	0.0450 mL	0.2252 mL	0.4503 mL	2.2516 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 µL Tween 80 and mix well until fully clarified.

3) Add 450 µL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Related Tags: Aplidine chemical structure | Aplidine in vivo | Aplidine in vitro | Aplidine formula | Aplidine molecular weight