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Cisplatin

Cisplatin
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Purity:99.62%
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Cisplatin

Catalog No. T1564Cas No. 15663-27-1
Cisplatin (CDDP) is a DNA cross-linking agent. Cisplatin has antitumor activity and inhibits DNA synthesis by forming DNA adducts in cancer cells. Cisplatin also activates ferroptosis and induces autophagy.
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Pack SizePriceAvailabilityQuantity
50 mg$28In Stock
100 mg$42In Stock
500 mg$97In Stock
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Related Compound Libraries of "Cisplatin"

Product Introduction

Bioactivity
Description
Cisplatin (CDDP) is a DNA cross-linking agent. Cisplatin has antitumor activity and inhibits DNA synthesis by forming DNA adducts in cancer cells. Cisplatin also activates ferroptosis and induces autophagy.
In vitro
METHODS: Human non-small cell lung cancer cells A549, SKMES-1, MOR and H460 were treated with Cisplatin (0.001-100 μM) for 72 h, and cell growth inhibition was detected by MTT.
RESULTS: Cisplatin dose-dependently inhibited the cell growth of A549, SKMES-1, MOR and H460 cells with IC50 of 1.58 µM, 4.09 µM, 6.39 µM and 5.72 µM, respectively. [1]
METHODS: Human breast cancer MCF-7 and MDA-MB-231 were treated with Cisplatin (2-10 μg/mL) for 48 h, and the expression levels of target proteins were detected by Western Blot.
RESULTS: Cisplatin dose-dependently induced increased levels of apoptosis-related proteins cleaved-caspase 3 and cleaved-PARP in MCF-7 and MDA-MB-231 cells. [2]
METHODS: Mesothelioma cells JU77, LO68 and ONE58 were treated with Cisplatin (5-100 μg/mL) for 24 h, and mitochondrial membrane potential (MMP) was detected using JC-1 dye.
RESULTS: Cisplatin dose-dependently decreased MMP and inhibited mitochondrial function in JU77, LO68 and ONE58 cells. [3]
In vivo
METHODS: To assay anti-tumor activity in vivo, Cisplatin (5 mg/kg/6 days) and Chloroquine (13 mg/kg/day) were intraperitoneally injected into BALB/c nude mice bearing hypopharyngeal squamous cell carcinoma tumors (HSCC) FaDu for eighteen days.
RESULTS: Cisplatin treatment significantly inhibited HSCC tumor growth. Chloroquine inhibited autophagy and increased Cisplatin-induced apoptosis, which enhanced the efficacy of Cisplatin, resulting in reduced tumor growth and prolonged survival in mice. [4]
METHODS: To attenuate nephrotoxicity induced by Cisplatin treatment, Cisplatin (3-6 mg/kg/3 days) was administered intraperitoneally and Cilastatin (100 mg/kg/day) subcutaneously to BALB/c mice with human lung adenocarcinoma tumor A549 for seven days.
RESULTS: Cilastatin can reduce the induced nephrotoxicity of Cisplatin without affecting its antitumor effects. [5]
Cell Research
Rabbit renal proximal tubules were isolated using the iron oxide perfusion method and grown in 35-mm tissue culture dishes under improved conditions as described previously. The cell culture medium was a 1:1 mixture of Dulbecco's modified Eagle's medium/Ham's F-12 (without D-glucose, phenol red, or sodium pyruvate) supplemented with 15 mM HEPES buffer, 2.5 mM L-glutamine, 1 μM pyridoxine HCl, 15 mM sodium bicarbonate, and 6 mM lactate. Hydrocortisone (50 nM), selenium (5 ng/ml), human transferrin (5 μg/ml), bovine insulin (10 nM), and L-ascorbic acid-2-phosphate (50 μM) were added to fresh culture medium immediately before daily media change. In general, confluent RPTCs were treated with inhibitors or diluent control [typically DMSO at 0.1% (v/v)] for 30 min before treatment with cisplatin. Aliquots of RPTCs were used for various assays as detailed below [1].
Animal Research
Mice were divided randomly into three groups (Control, Cisplatin and Cisplatin+HemoHIM), and each group consisted of twenty mice. B16F0 melanoma (5 × 10^5 cells/mouse) was inoculated into subcutaneous femoral left region of mice at 3 days before an initial injection of cisplatin. Cisplatin was injected intraperitoneally at 4 mg/kg body weight (B.W.) on day 0, 7 and 14 (total three injections). Experimental group was intubated with HemoHIM at a final concentration of 100 mg/kgB.W. by everyday from day -1 to day 16, while the control group received only water. On day 17 after initial injection of cisplatin, all mice of each group were experimented, respectively, to evaluate tumor weight or tumor size. The tumor size was calculated as follows: tumor size = ab^2/2, where a and b are the larger and smaller diameters, respectively [3].
Aliascis-Diaminodichloroplatinum, CDDP
Chemical Properties
Molecular Weight300.04
FormulaCl2H6N2Pt
Cas No.15663-27-1
Storage & Solubility Information
Storagekeep away from direct sunlight|Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Solubility Information
H2O: 1 mg/mL (3.3 mM), Heating at 50℃ is recommended.(DMSO can inactivate Cisplatin's activity.)
DMF: 20 mg/mL (66.7 mM)
Solution Preparation Table
DMF/H2O
1mg5mg10mg50mg
1 mM3.3329 mL16.6644 mL33.3289 mL166.6444 mL
DMF
1mg5mg10mg50mg
5 mM0.6666 mL3.3329 mL6.6658 mL33.3289 mL
10 mM0.3333 mL1.6664 mL3.3329 mL16.6644 mL
20 mM0.1666 mL0.8332 mL1.6664 mL8.3322 mL
50 mM0.0667 mL0.3333 mL0.6666 mL3.3329 mL
100 mM0.0333 mL0.1666 mL0.3333 mL1.6664 mL

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