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Catalog No. T3088   CAS 128-53-0
Synonyms: 1-Ethyl-1H-pyrrole-2,5-dione, Ethylmaleimide, NEM

N-Ethylmaleimide (NEM) is a sulfhydryl reagent that is widely used in experimental biochemical studies.

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N-Ethylmaleimide Chemical Structure
N-Ethylmaleimide, CAS 128-53-0
Pack Size Availability Price/USD Quantity
10 mg In stock $ 39.00
25 mg In stock $ 62.00
100 mg In stock $ 82.00
200 mg In stock $ 98.00
500 mg In stock $ 158.00
1 g In stock $ 236.00
1 mL * 10 mM (in DMSO) In stock $ 43.00
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Purity: 98%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description N-Ethylmaleimide (NEM) is a sulfhydryl reagent that is widely used in experimental biochemical studies.
Synonyms 1-Ethyl-1H-pyrrole-2,5-dione, Ethylmaleimide, NEM
Molecular Weight 125.13
Formula C6H7NO2
CAS No. 128-53-0


Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

5% DMSO+95% Saline: 1.2 mg/mL (9.59 mM)

DMSO: 60 mg/mL (479.5 mM)

TargetMolReferences and Literature

1. Chen YQ, et al. J Biophotonics. 2014 Aug;7(8):647-55.


1. Hussain M, Lu Y, Tariq M, et al. A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism. Iscience. 2022, 25(7): 104591. 2. Ren W, Xu Z, Chang Y, et al.Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1.Nature Communications.2024, 15(1): 9.

Related compound libraries

This product is contained In the following compound libraries:
Inhibitor Library Highly Selective Inhibitor Library Ubiquitination Compound Library Covalent Inhibitor Library FDA-Approved & Pharmacopeia Drug Library Cell Cycle Compound Library NO PAINS Compound Library Bioactive Compound Library DNA Damage & Repair Compound Library Target-Focused Phenotypic Screening Library

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An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
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The answer of 19.713 mg appears in the Mass box


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Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

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An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
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The answer of 100 microliter (0.1 ml) appears in the Volume (start) box


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Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.


Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.


N-Ethylmaleimide 128-53-0 Cell Cycle/Checkpoint DNA Damage/DNA Repair Proteases/Proteasome Ubiquitination Cysteine Protease DUB Deubiquitinase 1-Ethyl-1H-pyrrole-2,5-dione inhibit VSMC Apoptosis DUBs cysteine residue NEthylmaleimide N Ethylmaleimide Ethylmaleimide phosphate transport sulfhydryl Cathepsin NEM Inhibitor modify inhibitor