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Dioscin

Catalog No. T6473 CAS 19057-60-4

Synonyms: CCRIS 4123, Collettiside III, Saponin

Dioscin (Collettiside III) is a saponin with antitumor activities.

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Dioscin, CAS 19057-60-4

Pack Size	Availability	Price/USD	Quantity
20 mg	In stock	$ 50.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.85%

Purity: 98.35%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Dioscin (Collettiside III) is a saponin with antitumor activities.
In vitro	The chemical compound exhibits an LD50 value of greater than 300 mg/kg in mice, indicating the dosage at which half the population of test subjects succumbed to the compound's effects exceeded 300 mg/kg.
In vivo	Dioscin induces apoptosis by downregulating the activity of caspase-8 and upregulating the activity of caspase-9 in HeLa cells. It inhibits the growth of the MDA-MB-435, H14, HL60, and HeLa cell lines, with IC50 values of 2.6, 0.8, 7.5, and 4.5 μM, respectively.
Kinase Assay	Rat liver cytosol is prepared from a separate piece of liver from rat. Assays are carried out in a total volume of 0.1 mL containing 10 mg of cytosolic protein, and components according to Rilling. All reaction components (except IPP) are mixed and kept on ice for 15 min. Reactions are initiated by the addition of [14C]IPP and incubation at 37°C. Reactions are stopped after 5 min by addition of 0.4 mL MeOH/HCl (4/1, by vol.) and the samples are incubated a further 15 min to hydrolyze the allylic pyrophosphates to petroleum ether-extractable products. Following addition of 0.5 mL water and 1 mL petroleum ether, 50% of the upper (petroleum ether-extractable) phase is taken for liquid scintillation analysis. Preliminary experiments indicated that the reaction is linear with time and protein under these conditions, and no more than 10% of the substrate is consumed.
Source	Natural Products > Dioscoreaceae > Dioscorea

Synonyms	CCRIS 4123, Collettiside III, Saponin
Molecular Weight	869.04
Formula	C45H72O16
CAS No.	19057-60-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 100 mg/mL (115.06 mM)

Ethanol: 22 mg/mL (25.31 mM)

References and Literature

1. Wang Z, et al. Biol Pharm Bull, 2001, 24(2), 159-162. 2. Cai J, et al. Biol Pharm Bull. 2002, 25(2), 193-196.

Citations

1. An Y, Zhu J, Wang X, et al. Oridonin Delays Aging Through the AKT Signaling Pathway. Frontiers in Pharmacology. 2022: 1823 2. Li H, Pang B, Nie B, et al. Dioscin promotes autophagy by regulating the AMPK-mTOR pathway in ulcerative colitis. Immunopharmacology and Immunotoxicology. 2022: 1-9.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Anti-Cancer Approved Drug Library Drug Repurposing Compound Library Anti-Cancer Drug Library Human Metabolite Library Natural Product Library Natural Product Library for HTS Anti-COVID-19 Traditional Chinese Medicine Compound Library Saccharide and Glycoside Natural Product Library Approved Drug Library

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Dioscin 19057-60-4 Apoptosis Autophagy CCRIS 4123 Collettiside III CCRIS-4123 Inhibitor Saponin CCRIS4123 inhibit inhibitor

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