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Dioscin

Name: Dioscin
Brand: TargetMol
SKU: T6473
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T6473Cas No. 19057-60-4

Alias Collettiside III, CCRIS 4123

Dioscin (Collettiside III) is a saponin with antitumor activities.

Dioscin

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Purity: 99.87%

Catalog No. T6473Alias Collettiside III, CCRIS 4123Cas No. 19057-60-4

Dioscin (Collettiside III) is a saponin with antitumor activities.

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$30	In Stock	In Stock
10 mg	$37	In Stock	In Stock
25 mg	$56	In Stock	In Stock
50 mg	$78	In Stock	In Stock
100 mg	$113	In Stock	In Stock
200 mg	$163	In Stock	In Stock
500 mg	$273	-	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:99.87%

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Dioscin (Collettiside III) is a saponin with antitumor activities.
In vitro	The chemical compound exhibits an LD50 value of greater than 300 mg/kg in mice, indicating the dosage at which half the population of test subjects succumbed to the compound's effects exceeded 300 mg/kg.
In vivo	Dioscin induces apoptosis by downregulating the activity of caspase-8 and upregulating the activity of caspase-9 in HeLa cells. It inhibits the growth of the MDA-MB-435, H14, HL60, and HeLa cell lines, with IC50 values of 2.6, 0.8, 7.5, and 4.5 μM, respectively.
Kinase Assay	Rat liver cytosol is prepared from a separate piece of liver from rat. Assays are carried out in a total volume of 0.1 mL containing 10 mg of cytosolic protein, and components according to Rilling. All reaction components (except IPP) are mixed and kept on ice for 15 min. Reactions are initiated by the addition of [14C]IPP and incubation at 37°C. Reactions are stopped after 5 min by addition of 0.4 mL MeOH/HCl (4/1, by vol.) and the samples are incubated a further 15 min to hydrolyze the allylic pyrophosphates to petroleum ether-extractable products. Following addition of 0.5 mL water and 1 mL petroleum ether, 50% of the upper (petroleum ether-extractable) phase is taken for liquid scintillation analysis. Preliminary experiments indicated that the reaction is linear with time and protein under these conditions, and no more than 10% of the substrate is consumed.
Synonyms	Collettiside III, CCRIS 4123

Chemical Properties

Molecular Weight	869.04
Formula	C₄₅H₇₂O₁₆
Cas No.	19057-60-4
Smiles	C[C@H]1[C@H]2[C@H](C[C@H]3[C@@H]4CC=C5C[C@H](CC[C@@]5(C)[C@H]4CC[C@@]23C)O[C@@H]2O[C@H](CO)[C@@H](O[C@@H]3O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O)[C@H](O)[C@H]2O[C@@H]2O[C@@H](C)[C@H](O)[C@@H](O)[C@H]2O)O[C@@]11CC[C@@H](C)CO1
Relative Density.	1.39 g/cm3 (Predicted)

Storage & Solubility Information

Storage

keep away from direct sunlight,keep away from moisture,store at low temperature | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (115.07 mM), Sonication is recommended.

Ethanol: 22 mg/mL (25.32 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (4.6 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	1.1507 mL	5.7535 mL	11.5070 mL	57.5348 mL
5 mM	0.2301 mL	1.1507 mL	2.3014 mL	11.5070 mL
10 mM	0.1151 mL	0.5753 mL	1.1507 mL	5.7535 mL
20 mM	0.0575 mL	0.2877 mL	0.5753 mL	2.8767 mL

DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0230 mL	0.1151 mL	0.2301 mL	1.1507 mL
100 mM	0.0115 mL	0.0575 mL	0.1151 mL	0.5753 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc