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BAM7

Catalog No. T2453 CAS 331244-89-4

BAM 7 is a direct and specific activator of proapoptotic Bax (EC50: 3.3 μM).

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BAM7, CAS 331244-89-4

Pack Size	Availability	Price/USD
1 mg	In stock	$ 32.00
5 mg	In stock	$ 73.00
10 mg	In stock	$ 103.00
25 mg	In stock	$ 217.00
50 mg	In stock	$ 395.00
100 mg	In stock	$ 593.00
1 mL * 10 mM (in DMSO)	In stock	$ 82.00

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Purity: 99.23%

Purity: 98.68%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	BAM 7 is a direct and specific activator of proapoptotic Bax (EC50: 3.3 μM).
Targets&IC50	Bax:3.3 mM(EC50)
In vivo	In vitro experiments show that BAM7 induces BAX-mediated pore formation, BAX oligomerization, and BAX-dependent cell death. By activating BAX within cells, BAM7 selectively triggers BAX-mediated apoptosis. Its efficacy is limited to cells containing BAX, where it induces the biochemical and morphological hallmarks of BAX-mediated apoptosis. BAM7 binds directly to the N-terminal BH3 domain of BAX, facilitating interaction at the surface and BAX activation via the BIM BH3 helix. Moreover, BAM7 dose-dependently and temporally triggers the conversion of BAX from monomers to oligomers.
Kinase Assay	Fluorescence polarization binding assays: Direct binding curves are first generated by incubating FITC-BIM SAHB (50 nM) with serial dilutions of fulllength BAX, BCL-XLΔC, MCL-1ΔNΔC, BFL-1/A1ΔC or BAKΔC and fluorescence polarization measured at 20 minutes on a SpectraMax M5 microplate reader. For competition assays, a serial dilution of small molecule or acetylated BIM SAHB (Ac-BIM SAHB) is combined with FITC-BIM SAHB (50 nM), followed by the addition of recombinant protein at ~EC75 concentration, as determined by the direct binding assay (BAX, BAKΔC: 500 nM; BCL-XLΔC, MCL-1ΔNΔC, BFL-1/A1ΔC: 200 nM). Fluorescence polarization is measured at 20 minutes and IC50 values calculated by nonlinear regression analysis of competitive binding curves using Prism software.
Cell Research	MEFs (2.5 &times 103 cells per well) are seeded in 96-well opaque plates for 18-24 h and then incubated with serial dilutions of BAM7, ANA-BAM16 or vehicle (0.15% (v/v) DMSO) in DMEM at 37 °C in a final volume of 100 μL. Cell viability is assayed at 24 h by addition of CellTiter-Glo reagent according to the manufacturer's protocol, and luminescence is measured using a SpectraMax M5 microplate reader. Viability assays are performed in at least triplicate, and the data are normalized to vehicle-treated control wells.(Only for Reference)

Molecular Weight	405.47
Formula	C21H19N5O2S
CAS No.	331244-89-4

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 4.1 mg/mL (10 mM)

References and Literature

1. Gavathiotis E, et al. Nat Chem Biol, 2012, 8(7), 639-645.

Related compound libraries

This product is contained In the following compound libraries：

Mitochondria-Targeted Compound Library PPI Inhibitor Library Cuproptosis Compound Library Bioactive Compound Library Bioactive Compounds Library Max Apoptosis Compound Library Anti-Cancer Compound Library Target-Focused Phenotypic Screening Library

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Keywords

BAM7 331244-89-4 Apoptosis BCL Bcl-2 Family BAM-7 Inhibitor inhibit BAM 7 inhibitor

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