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Phenformin hydrochloride

Name: Phenformin hydrochloride
Brand: TargetMol
SKU: T1453
Price: 42 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1453Cas No. 834-28-6

Alias Phenformin HCl, Phenethylbiguanide hydrochloride

Phenformin hydrochloride (Phenformin HCl) is an agent belonging to the biguanide class of antidiabetics with antihyperglycemic activity.

Phenformin hydrochloride

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Purity: 99.80%

Catalog No. T1453Alias Phenformin HCl, Phenethylbiguanide hydrochlorideCas No. 834-28-6

Phenformin hydrochloride (Phenformin HCl) is an agent belonging to the biguanide class of antidiabetics with antihyperglycemic activity.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$42	-	In Stock
5 g	$66	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.80%

Appearance:Solid

Color:White

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Phenformin hydrochloride (Phenformin HCl) is an agent belonging to the biguanide class of antidiabetics with antihyperglycemic activity.
In vitro	In H441 cells, phenformin increased AMPK activity while decreasing basal ion transport. In isolated hearts, Phenformin increased AMPK activity and phosphorylation. Phenformin significantly induced apoptosis in LKB1-deficient NSCLC cell lines.
In vivo	In H441 cells, phenformin increased AMPK activity while decreasing basal ion transport. In isolated hearts, Phenformin increased AMPK activity and phosphorylation. Phenformin significantly induced apoptosis in LKB1-deficient NSCLC cell lines.
Kinase Assay	Total AMPK activity is measured using the method of Dagher et al. AMPK activity is quantified in the resuspended pellet as incorporation of?32P from [γ-32P]ATP (10 GBq/mmol) into a synthetic peptide with the specific target sequence for AMPK, the SAMS peptide. Radioactivity is measured using a liquid scintillation counter. Protein content in the solution containing the resupended (NH4)2SO4 pellet is determined using the Bradford method.
Synonyms	Phenformin HCl, Phenethylbiguanide hydrochloride

Chemical Properties

Molecular Weight	241.72
Formula	C₁₀H₁₆ClN₅
Cas No.	834-28-6
Smiles	Cl.NC(=N)NC(=N)NCCc1ccccc1
Relative Density.	1.24 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (1034.25 mM), Sonication is recommended.

H2O: 44 mg/mL (182.03 mM), Sonication is recommended.

Ethanol: 10 mg/mL (41.37 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (4.14 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1370 mL	20.6851 mL	41.3702 mL	206.8509 mL
5 mM	0.8274 mL	4.1370 mL	8.2740 mL	41.3702 mL
10 mM	0.4137 mL	2.0685 mL	4.1370 mL	20.6851 mL
20 mM	0.2069 mL	1.0343 mL	2.0685 mL	10.3425 mL

H2O/DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0827 mL	0.4137 mL	0.8274 mL	4.1370 mL
100 mM	0.0414 mL	0.2069 mL	0.4137 mL	2.0685 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc