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Phenformin hydrochloride

Name: Phenformin hydrochloride
Brand: TargetMol
SKU: T1453
Price: 42 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T1453Cas No. 834-28-6

Alias Phenformin HCl, Phenethylbiguanide hydrochloride

Phenformin hydrochloride (Phenformin HCl) is an agent belonging to the biguanide class of antidiabetics with antihyperglycemic activity.

Phenformin hydrochloride

🥰Excellent

Purity: 99.80%

Catalog No. T1453Alias Phenformin HCl, Phenethylbiguanide hydrochlorideCas No. 834-28-6

Phenformin hydrochloride (Phenformin HCl) is an agent belonging to the biguanide class of antidiabetics with antihyperglycemic activity.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$42	-	In Stock
5 g	$66	-	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.80%

Resource Download

COA HNMR LCMS

Product Introduction

Bioactivity

Description	Phenformin hydrochloride (Phenformin HCl) is an agent belonging to the biguanide class of antidiabetics with antihyperglycemic activity.
In vitro	In H441 cells, phenformin increased AMPK activity while decreasing basal ion transport. In isolated hearts, Phenformin increased AMPK activity and phosphorylation. Phenformin significantly induced apoptosis in LKB1-deficient NSCLC cell lines.
In vivo	In H441 cells, phenformin increased AMPK activity while decreasing basal ion transport. In isolated hearts, Phenformin increased AMPK activity and phosphorylation. Phenformin significantly induced apoptosis in LKB1-deficient NSCLC cell lines.
Kinase Assay	Total AMPK activity is measured using the method of Dagher et al. AMPK activity is quantified in the resuspended pellet as incorporation of?32P from [γ-32P]ATP (10 GBq/mmol) into a synthetic peptide with the specific target sequence for AMPK, the SAMS peptide. Radioactivity is measured using a liquid scintillation counter. Protein content in the solution containing the resupended (NH4)2SO4 pellet is determined using the Bradford method.
Synonyms	Phenformin HCl, Phenethylbiguanide hydrochloride

Chemical Properties

Molecular Weight	241.72
Formula	C₁₀H₁₆ClN₅
Cas No.	834-28-6
Smiles	Cl.NC(=N)NC(=N)NCCc1ccccc1
Relative Density.	1.24 g/cm3

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (248.22 mM), Sonication is recommended.

H2O: 44 mg/mL (182.03 mM), Sonication is recommended.

Ethanol: 10 mg/mL (41.37 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween-80+45% Saline: 1 mg/mL (4.14 mM), Sonication is recommeded.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	4.1370 mL	20.6851 mL	41.3702 mL	206.8509 mL
5 mM	0.8274 mL	4.1370 mL	8.2740 mL	41.3702 mL
10 mM	0.4137 mL	2.0685 mL	4.1370 mL	20.6851 mL
20 mM	0.2069 mL	1.0343 mL	2.0685 mL	10.3425 mL

H2O/DMSO

	1mg	5mg	10mg	50mg
50 mM	0.0827 mL	0.4137 mL	0.8274 mL	4.1370 mL
100 mM	0.0414 mL	0.2069 mL	0.4137 mL	2.0685 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc