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Anti-AMPK alpha 1 Antibody (2A443)
😃Good
Catalog No. TMAB-00114
Anti-AMPK alpha 1 Antibody (2A443) is a Mouse antibody targeting AMPK alpha 1. Anti-AMPK alpha 1 Antibody (2A443) can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AMPK alpha 1 Antibody (2A443)
Anti-AMPK alpha 1 Antibody (2A443)
😃Good
Catalog No. TMAB-00114
Anti-AMPK alpha 1 Antibody (2A443) is a Mouse antibody targeting AMPK alpha 1. Anti-AMPK alpha 1 Antibody (2A443) can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $221 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $529 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Mouse Monoclonal Application: WB,IHC-P,IHC-Fr,IF Reactivity: Human,Mouse,Rat |
| Ig Type | IgG1 |
| Clone | 2A443 |
| Reactivity | Human,Mouse,Rat |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha-1) Monoclonal Antibody, Unconjugated (TMAB-00114) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Sample: Lane 1: Rat Heart Lysates Lane 2: Rat Brain Lysates Lane 3: Rat Skeletal muscle Lysates Primary: Anti-AMPK alpha-1 (TMAB-00114) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa |
| Application | WB,IHC-P,IHC-Fr,IF |
| Recommended Dose | WB: 1:500-1000; IHC-P: 1:200-800; IHC-Fr: 1:200-500; IF: 1:200-500 |
| Antibody Type | Monoclonal |
| Host Species | Mouse |
| Subcellular Localization | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
| Construction | Hybridoma Monoclonal Antibody |
| Purification | Protein G purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant Protein: human AMPK alpha-1 Protein |
| Antigen Species | Human |
| Gene ID | 5562 |
| Biology Area | Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Cancer,Neurodegenerative disease,Amyloid,Other Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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