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Anti-AMPK alpha 1 Antibody (2A443) is a Mouse antibody targeting AMPK alpha 1. Anti-AMPK alpha 1 Antibody (2A443) can be used in WB,IHC-P,IHC-Fr,IF.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μL | $221 | 7-10 days | |
100 μL | $372 | 7-10 days | |
200 μL | $529 | 7-10 days |
Description | Anti-AMPK alpha 1 Antibody (2A443) is a Mouse antibody targeting AMPK alpha 1. Anti-AMPK alpha 1 Antibody (2A443) can be used in WB,IHC-P,IHC-Fr,IF. |
Alias | wu:fa94c10, SNF1-like protein AMPK, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA1, PRKAA 1, MGC57364, MGC33776, kinase AMPK alpha1, im:7154392, hormone sensitive lipase kinase, HMG CoA reductase kinase, EC 2.7.11.1, cb116, C130083N04Rik, AMPKα1, AMPKα 1, AMPKalpha1, AMPKa1, AMPK-a, AMPK1, AMPK α1, AMPK α 1, AMPK alpha-1, AMPK alpha 1 chain, AMPK a1, AMPK 1, AMPK, AMP-activated protein kinase, catalytic, alpha-1, AMP-activate kinase alpha 1 subunit, AL024255, AI450832, AI194361, acetyl CoA carboxylase kinase, AAPK1, 5' AMP activated protein kinase catalytic subunit alpha 1, 5 AMP activated protein kinase catalytic alpha 1 chain, 5 AMP activated protein kinase alpha 1catalytic subunit |
Ig Type | IgG1 |
Clone | 2A443 |
Reactivity | Human,Mouse,Rat |
Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha-1) Monoclonal Antibody, Unconjugated (TMAB-00114) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructionsand DAB staining. 2. Sample: Lane 1: Rat Heart Lysates Lane 2: Rat Brain Lysates Lane 3: Rat Skeletal muscle Lysates Primary: Anti-AMPK alpha-1 (TMAB-00114) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 64 kDa Observed band size: 64 kDa |
Application | WB,IHC-P,IHC-Fr,IF |
Recommended Dose | WB: 1:500-1000; IHC-P: 1:200-800; IHC-Fr: 1:200-500; IF: 1:200-500 |
Antibody Type | Monoclonal |
Host Species | Mouse |
Subcellular Localization | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
Construction | Hybridoma Monoclonal Antibody |
Purification | Protein G purified |
Appearance | Liquid |
Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
Research Background | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
Immunogen | Recombinant Protein: human AMPK alpha-1 Protein |
Antigen Species | Human |
Gene ID | |
Biology Area | Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Cancer,Neurodegenerative disease,Amyloid,Other Kinases |
Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
Transport | Shipping with blue ice. |
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