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Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody
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Catalog No. TMAB-01388
Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPK alpha 1/2 (Thr183, 172). Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody
Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody
😃Good
Catalog No. TMAB-01388
Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPK alpha 1/2 (Thr183, 172). Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $372 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Antibody Type: Rabbit Polyclonal Application: IHC-P,IHC-Fr,IF,FCM Reactivity: Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (TMAB-01388) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (TMAB-01388) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (TMAB-01388) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. |
| Application | IHC-P,IHC-Fr,IF,FCM |
| Recommended Dose | IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters. |
| Construction | Hybridoma Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| Immunogen | KLH conjugated Synthesised phosphopeptide: human AMPK alpha 1 around the phosphorylation site of Thr183 |
| Antigen Species | Human |
| Gene ID | 5562 |
| Biology Area | Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Cancer,Neurodegenerative disease,Amyloid,Other Kinases |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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