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Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody

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Catalog No. TMAB-01388
Alias α-1, Tau-protein kinase PRKAA1, Protein kinase AMP activated alpha 2 catalytic subunit, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA2, PRKAA1, PRKAA, p-AMPK alpha 1/2 (Thr183, 172), p-AMPK alpha 1/2 (T183, 172), Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, AMPK-α-1, AMPKα1, AMPKα 1, AMPK2, AMPK1, AMPK α1, AMPK α 1, AMPK subunit alpha-1, AMPK AMPK-α1, AMPK alpha 2 chain, AMPK alpha 1/2 (p-Thr183, 172), AMPK alpha 1/2 (p-T183, 172), AMPK alpha 1 chain, AMPK, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1

Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPK alpha 1/2 (Thr183, 172). Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.

Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody

Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody

😃Good
Catalog No. TMAB-01388Alias α-1, Tau-protein kinase PRKAA1, Protein kinase AMP activated alpha 2 catalytic subunit, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA2, PRKAA1, PRKAA, p-AMPK alpha 1/2 (Thr183, 172), p-AMPK alpha 1/2 (T183, 172), Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, AMPK-α-1, AMPKα1, AMPKα 1, AMPK2, AMPK1, AMPK α1, AMPK α 1, AMPK subunit alpha-1, AMPK AMPK-α1, AMPK alpha 2 chain, AMPK alpha 1/2 (p-Thr183, 172), AMPK alpha 1/2 (p-T183, 172), AMPK alpha 1 chain, AMPK, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1
Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPK alpha 1/2 (Thr183, 172). Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5287-10 days
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Product Introduction

Bioactivity
Description
Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody is a Rabbit antibody targeting Phospho-AMPK alpha 1/2 (Thr183, 172). Anti-Phospho-AMPK alpha 1/2 (Thr183, 172) Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF,FCM.
Aliasα-1, Tau-protein kinase PRKAA1, Protein kinase AMP activated alpha 2 catalytic subunit, Protein kinase AMP activated alpha 1 catalytic subunit, PRKAA2, PRKAA1, PRKAA, p-AMPK alpha 1/2 (Thr183, 172), p-AMPK alpha 1/2 (T183, 172), Hydroxymethylglutaryl-CoA reductase kinase, HMGCR kinase, AMPK-α-1, AMPKα1, AMPKα 1, AMPK2, AMPK1, AMPK α1, AMPK α 1, AMPK subunit alpha-1, AMPK AMPK-α1, AMPK alpha 2 chain, AMPK alpha 1/2 (p-Thr183, 172), AMPK alpha 1/2 (p-T183, 172), AMPK alpha 1 chain, AMPK, Acetyl-CoA carboxylase kinase, ACACA kinase, AAPK2, AAPK1, 5'-AMP-activated protein kinase catalytic subunit alpha-1
Ig Type
IgG
Reactivity
Human,Mouse,Rat (predicted:Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep)
Verified Activity
1. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (TMAB-01388) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (TMAB-01388) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172)) Polyclonal Antibody, Unconjugated (TMAB-01388) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Application
IHC-P,IHC-Fr,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500; FCM: 1ug/test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThe protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Antigen Details
Immunogen
KLH conjugated Synthesised phosphopeptide: human AMPK alpha 1 around the phosphorylation site of Thr183
Antigen Species
Human
Gene ID
Biology Area
Integration of energy metabolism,Response to hypoxia,Metabolism,Integration of energy,Fatty acids,Hypoxia,Fatty acid oxidation,Cancer,Neurodegenerative disease,Amyloid,Other Kinases
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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