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Indole-3-carbinol

Name: Indole-3-carbinol
Brand: TargetMol
SKU: T2947
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2947Cas No. 700-06-1

Alias Indole-3-Methanol, I3C, 3-Indolemethanol

Indole-3-carbinol (I3C), a naturally occurring, orally available cleavage product of the glucosinolate glucobrassicanin, inhibits NF-κB and IκBα kinase activation.

Indole-3-carbinol

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Purity: 98.59%

Catalog No. T2947Alias Indole-3-Methanol, I3C, 3-IndolemethanolCas No. 700-06-1

Indole-3-carbinol (I3C), a naturally occurring, orally available cleavage product of the glucosinolate glucobrassicanin, inhibits NF-κB and IκBα kinase activation.

Pack Size	Price	USA Warehouse	Global Warehouse	Quantity
1 g	$29	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

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Batch Information

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Purity:98.59%

Appearance:Solid

Color:White

Resource Download

COA HNMR HPLC

Product Introduction

Indole-3-carbinol AI Summary

Indole-3-carbinol demonstrates a diverse range of bioactivities and potential therapeutic applications across various biological targets and pathways. It shows notable sulfonation activity against Arylsulfotransferase (AST IV), with an apparent Michaelis constant (Km) of 29000.0 nM, a maximum velocity (Vmax) of 33.2 nM min^-1, and a catalytic efficiency (kcat/Km) of 38.9. The compound exhibits inhibitory properties in numerous enzymatic and cellular assays such as Schistosoma Mansoni Peroxiredoxins, Anthrax Lethal Toxin Internalization, AmpC Beta-Lactamase, and several other targets related to disease processes. Additionally, it inhibits the uptake of sodium fluorescein in OATP1B1 and OATP1B3-transfected CHO cells, showing substantial inhibition rates of 88.32% and 121.9%, respectively, at 10 µM. In antifungal assays, Indole-3-carbinol inhibits the mycelial growth of Leptosphaeria maculans on potato dextrose agar with increasing inhibitory percentages at higher concentrations: 19.0% at 0.10 mM, 27.0% at 0.20 mM, and 52.0% at 0.50 mM, with a half-life of more than 72 hours in water. It also exhibits mild inhibition of acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) at a concentration of 2 mg/ml, showing 4.82% and 9.11% inhibition, respectively. The compound shows antiproliferative and cytotoxic activities against several human cell lines, including U87MG and 1321N1, with IC50 values of 526,000.0 nM and 309,000.0 nM respectively, and against IN1472, U251, U87, IN1528, and IN1760 cells, with EC50 values ranging from 290,000 nM to 1,580,000 nM. Furthermore, Indole-3-carbinol demonstrates antiviral activity, inhibiting SARS-CoV-2 induced cytotoxicity in Caco-2 and VERO-6 cells at various concentrations, showing inhibition rates up to 1.16%. However, its inhibitory activity on SARS-CoV-2 3CL-Pro protease at 20 µM exhibits inconsistent results, with percentages of -27.37% and 5.975%. Lastly, Indole-3-carbinol also acts as an HDAC6 inhibitor, with inhibition percentages of 2.93% and 1.64% in enzymatic assays with commercial and custom peptide substrates, respectively. This suggests its potential utility across multiple disease models and therapeutic areas..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	Indole-3-carbinol (I3C), a naturally occurring, orally available cleavage product of the glucosinolate glucobrassicanin, inhibits NF-κB and IκBα kinase activation.
Synonyms	Indole-3-Methanol, I3C, 3-Indolemethanol

Chemical Properties

Molecular Weight	147.17
Formula	C₉H₉NO
Cas No.	700-06-1
Smiles	C(O)C=1C=2C(NC1)=CC=CC2
Relative Density.	1.1135 g/cm3 (Estimated)

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 250 mg/mL (1698.72 mM), Sonication is recommended.

Ethanol: 28 mg/mL (190.26 mM), Sonication is recommended.

H2O: 7 mg/mL (47.56 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (6.79 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	6.7949 mL	33.9743 mL	67.9486 mL	339.7432 mL
5 mM	1.3590 mL	6.7949 mL	13.5897 mL	67.9486 mL
10 mM	0.6795 mL	3.3974 mL	6.7949 mL	33.9743 mL
20 mM	0.3397 mL	1.6987 mL	3.3974 mL	16.9872 mL

Ethanol/DMSO

	1mg	5mg	10mg	50mg
50 mM	0.1359 mL	0.6795 mL	1.3590 mL	6.7949 mL
100 mM	0.0679 mL	0.3397 mL	0.6795 mL	3.3974 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc