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Anti-AHR Polyclonal Antibody

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Catalog No. TMAB-00080
Alias Class E basic helix-loop-helix protein 76 (bHLHe76), bHLHe76, Aryl hydrocarbon receptor, AHR, Ah receptor

Anti-AHR Polyclonal Antibody is a Rabbit antibody targeting AHR. Anti-AHR Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.

Anti-AHR Polyclonal Antibody

Anti-AHR Polyclonal Antibody

😃Good
Catalog No. TMAB-00080Alias Class E basic helix-loop-helix protein 76 (bHLHe76), bHLHe76, Aryl hydrocarbon receptor, AHR, Ah receptor
Anti-AHR Polyclonal Antibody is a Rabbit antibody targeting AHR. Anti-AHR Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
Pack SizePriceAvailabilityQuantity
50 μL$2207-10 days
100 μL$3727-10 days
200 μL$5297-10 days
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Product Introduction

Bioactivity
Description
Anti-AHR Polyclonal Antibody is a Rabbit antibody targeting AHR. Anti-AHR Polyclonal Antibody can be used in IHC-P,IHC-Fr,ICC/IF,IF,FCM.
AliasClass E basic helix-loop-helix protein 76 (bHLHe76), bHLHe76, Aryl hydrocarbon receptor, AHR, Ah receptor
Ig Type
IgG
Reactivity
Human,Rat (predicted:Mouse)
Verified Activity
1. HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (AHR) polyclonal Antibody, Unconjugated (TMAB-00080) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AHR Polyclonal Antibody, Unconjugated (TMAB-00080) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-AHR Polyclonal Antibody, Unconjugated (TMAB-00080) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Blank control: U937. Primary Antibody (green line): Rabbit Anti-AHR antibody (TMAB-00080)
Dilution: 2 μg/10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG.
Secondary Antibody: Goat anti-rabbit IgG-PE
Dilution: 1 μg/test.
Protocol
The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
Application
IHC-P,IHC-Fr,ICC/IF,IF,FCM
Recommended Dose
IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500; FCM: 2ug/Test
Antibody Type
Polyclonal
Host SpeciesRabbit
Subcellular LocalizationCytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus.
ConstructionHybridoma Polyclonal Antibody
PurificationProtein A purified
AppearanceLiquid
Formulation0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Research BackgroundThis gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons.
Antigen Details
Immunogen
KLH conjugated synthetic peptide: human AHR
Antigen Species
Human
Gene ID
Uniprot ID
Chemical Properties
Stability & Storage
Stability & StorageStore at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
TransportShipping with blue ice.

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

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