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Anti-AHR Polyclonal Antibody 2
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Catalog No. TMAB-00081
Alias Class E basic helix-loop-helix protein 76 (bHLHe76), bHLHe76, Aryl hydrocarbon receptor, AHR, Ah receptor
Anti-AHR Polyclonal Antibody 2 is a Rabbit antibody targeting AHR. Anti-AHR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
Anti-AHR Polyclonal Antibody 2
Anti-AHR Polyclonal Antibody 2
😃Good
Catalog No. TMAB-00081Alias Class E basic helix-loop-helix protein 76 (bHLHe76), bHLHe76, Aryl hydrocarbon receptor, AHR, Ah receptor
Anti-AHR Polyclonal Antibody 2 is a Rabbit antibody targeting AHR. Anti-AHR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 50 μL | $220 | 7-10 days | |
| 100 μL | $374 | 7-10 days | |
| 200 μL | $528 | 7-10 days |
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Product Introduction
Bioactivity
Antigen Details
Chemical Properties
| Description | Anti-AHR Polyclonal Antibody 2 is a Rabbit antibody targeting AHR. Anti-AHR Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Alias | Class E basic helix-loop-helix protein 76 (bHLHe76), bHLHe76, Aryl hydrocarbon receptor, AHR, Ah receptor |
| Ig Type | IgG |
| Reactivity | Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit,Sheep) |
| Verified Activity | 1. Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AHR) Polyclonal Antibody, Unconjugated (TMAB-00081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Paraformaldehyde-fixed, paraffin embedded (mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (AHR) Polyclonal Antibody, Unconjugated (TMAB-00081) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 3. Sample: Lane 1: Mouse Testis tissue lysates Lane 2: Rat Testis tissue lysates Lane 3: Human PC-3 cell lysates Primary: Anti-AHR (TMAB-00081) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 93 kDa Observed band size: 120 kDa |
| Application | |
| Recommended Dose | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| Antibody Type | Polyclonal |
| Host Species | Rabbit |
| Subcellular Localization | Cytoplasm. Nucleus. Initially cytoplasmic; upon binding with ligand and interaction with a HSP90, it translocates to the nucleus. |
| Construction | Polyclonal Antibody |
| Purification | Protein A purified |
| Appearance | Liquid |
| Formulation | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Research Background | This gene encodes a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Its ligands included a variety of aromatic hydrocarbons. |
| Immunogen | KLH conjugated synthetic peptide: human AHR |
| Antigen Species | Human |
| Gene ID | |
| Uniprot ID |
| Molecular Weight | Theoretical: 93 kDa. |
Stability & Storage
| Stability & Storage | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| Transport | Shipping with blue ice. |
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For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . 
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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