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BIX-01294

Name: BIX-01294
Brand: TargetMol
SKU: T7697
Price: 34 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T7697Cas No. 935693-62-2

BIX-01294 is an G9a Histone Methyltransferase inhibitor(IC50 : 1.9 μM).

BIX-01294

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Purity: 99.64%

Catalog No. T7697Cas No. 935693-62-2

BIX-01294 is an G9a Histone Methyltransferase inhibitor(IC50 : 1.9 μM).

Pack Size	Price	USA Warehouse	Global Warehouse
1 mg	$34	In Stock	In Stock
5 mg	$77	In Stock	In Stock
10 mg	$119	In Stock	In Stock
25 mg	$218	In Stock	In Stock
50 mg	$328	In Stock	In Stock
100 mg	$496	In Stock	In Stock
200 mg	$675	-	In Stock
1 mL x 10 mM (in DMSO)	$133	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.64%

Appearance:Solid

Color:White to Yellow

Resource Download

COA LCMS HNMR

Product Introduction

Bioactivity

Description	BIX-01294 is an G9a Histone Methyltransferase inhibitor(IC50 : 1.9 μM).
Targets&IC50	G9a:1.9 μM
In vitro	BIX-01294 induced caspase-dependent apoptosis in human bladder cancer cells.?BIX-01294 stimulates endoplasmic reticulum stress (ER stress) and up-regulated expression of PMAIP1 through DDIT3 up-regulation.?Furthermore, down-regulation of the deubiquitinase USP9X by BIX-01294 results in downstream reduction of MCL1 expression, leading to apoptosis eventually[1].
Cell Research	On the first day, cells were seeded in 96 well plates.?Twenty-four hours later, BIX-01294 was added at the indicated concentrations in each well.?After treatment by BIX-01294 for another 24 h, we used 10% TCA to immobilize the cells and then evaluated the living cell numbers using the sulforhodamine B assay as previously described[1]

Chemical Properties

Molecular Weight	490.64
Formula	C₂₈H₃₈N₆O₂
Cas No.	935693-62-2
Smiles	N(C=1C2=C(N=C(N1)N3CCN(C)CCC3)C=C(OC)C(OC)=C2)C4CCN(CC5=CC=CC=C5)CC4
Relative Density.	1.195 g/cm3 (Predicted)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 84.17 mg/mL (171.55 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 3.3 mg/mL (6.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0382 mL	10.1908 mL	20.3815 mL	101.9077 mL
5 mM	0.4076 mL	2.0382 mL	4.0763 mL	20.3815 mL
10 mM	0.2038 mL	1.0191 mL	2.0382 mL	10.1908 mL
20 mM	0.1019 mL	0.5095 mL	1.0191 mL	5.0954 mL
50 mM	0.0408 mL	0.2038 mL	0.4076 mL	2.0382 mL
100 mM	0.0204 mL	0.1019 mL	0.2038 mL	1.0191 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc