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Nicotinamide

Name: Nicotinamide
Brand: TargetMol
SKU: T0934
Price: 29 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T0934Cas No. 98-92-0

Alias Vitamin PP, Vitamin B3, Nicotinic acid amide, Niacinamide

Nicotinamide, a vitamin B3 derivative, is an inhibitor of SIRT1 (IC50=50-180 μM) and SIRT2 (IC50=2 μM). Nicotinamide can be used as a precursor of nicotinamide adenine dinucleotide or NAD+, which can be supplemented by dietary intake and can prevent or treat melanoglossia and pellagra.

Nicotinamide

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Purity: 99.86%

Catalog No. T0934Alias Vitamin PP, Vitamin B3, Nicotinic acid amide, NiacinamideCas No. 98-92-0

Pack Size	Price	USA Warehouse	Global Warehouse
2 g	$32	-	In Stock
5 g	$40	-	In Stock
10 g	$56	-	In Stock
25 g	$92	-	In Stock
1 mL x 10 mM (in DMSO)	$29	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.86%

Appearance:Solid

Color:White

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COA HNMR LCMS

Product Introduction

Bioactivity

Description	Nicotinamide, a vitamin B3 derivative, is an inhibitor of SIRT1 (IC50=50-180 μM) and SIRT2 (IC50=2 μM). Nicotinamide can be used as a precursor of nicotinamide adenine dinucleotide or NAD+, which can be supplemented by dietary intake and can prevent or treat melanoglossia and pellagra.
Targets&IC50	SIRT2:2 μM (EC50), SIRT1:50-180 μM, MDA-MB-436 cells:30.09 mM, HEK293T cells:43.8 μM, MDA-MB-231 cells:20.09 mM, RAW 264.7 cells:> 10 μM, MCF-7 cells:20.01 mM, Sir2Tm-D101N mutant:14.6 ± 3.4 mM, SK-MEL-28 cells:100 μM, BL21 cells:6.2 μM
In vitro	METHODS: A375, SK-MEL-28, and mouse B16-F10 cells were treated with Nicotinamide (0, 1, 20, and 50 mM) for 24 or 48 hours. RESULTS: Nicotinamide reduced cell numbers ina dose-dependent manner, with a strong inhibitory effect at 20mM and almost full effect at 50mM. [1]
In vivo	METHODS: To study the anti-tumor activity of Nicotinamide, mice were intraperitoneally injected with Nicotinamide (1500 and 1800 mg/Kg, 5 days a week). RESULTS: Nicotinamide inhibited tumor growth ina mouse model of metastatic melanoma. [1] METHODS: To study the antiviral activity of Nicotinamide, Nicotinamide (0-200 mg/kg, 5 days) was intraperitoneally injected into HBV transgenic mice. RESULTS: Nicotinamide inhibited HBV replication in HBV transgenic mice. [2]
Synonyms	Vitamin PP, Vitamin B3, Nicotinic acid amide, Niacinamide

Chemical Properties

Molecular Weight	122.12
Formula	C₆H₆N₂O
Cas No.	98-92-0
Smiles	NC(=O)C1=CN=CC=C1
Relative Density.	1.40 g/cm3

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 260 mg/mL (2129.05 mM), Sonication is recommended.

Ethanol: 23 mg/mL (188.34 mM), Sonication is recommended.

H2O: 122.7 mg/mL (1004.75 mM), Sonication is recommended.

In Vivo Formulation

Saline: 50 mg/mL (409.43 mM), Solution.

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5 mg/mL (40.94 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

Ethanol/H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	8.1887 mL	40.9433 mL	81.8867 mL	409.4333 mL
5 mM	1.6377 mL	8.1887 mL	16.3773 mL	81.8867 mL
10 mM	0.8189 mL	4.0943 mL	8.1887 mL	40.9433 mL
20 mM	0.4094 mL	2.0472 mL	4.0943 mL	20.4717 mL
50 mM	0.1638 mL	0.8189 mL	1.6377 mL	8.1887 mL
100 mM	0.0819 mL	0.4094 mL	0.8189 mL	4.0943 mL

Calculator

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc