Powder: -20°C for 3 years | In solvent: -80°C for 2 years
NVP-TAE226 is an effective FAK inhibitor (IC50: 5.5 nM) and most effective to Pyk2(IC50: 3.5 nM); 10- to 100-fold less effective against IGF-1R, InsR, c-Met, and ALK.
Pack Size | Availability | Price/USD | Quantity |
---|---|---|---|
1 mg | In stock | $ 41.00 | |
2 mg | In stock | $ 57.00 | |
5 mg | In stock | $ 103.00 | |
10 mg | In stock | $ 166.00 | |
25 mg | In stock | $ 277.00 | |
50 mg | In stock | $ 397.00 | |
100 mg | In stock | $ 597.00 | |
1 mL * 10 mM (in DMSO) | In stock | $ 103.00 |
Description | NVP-TAE226 is an effective FAK inhibitor (IC50: 5.5 nM) and most effective to Pyk2(IC50: 3.5 nM); 10- to 100-fold less effective against IGF-1R, InsR, c-Met, and ALK. |
Targets&IC50 | FAK:5.5 nM, PYK2:3.5 nM |
Kinase Assay | Kinase assay: Kinase activities are assayed in Buffer A or C at 30°C, at a final ATP concentration of 15 μM. Blank values are subtracted and activities calculated as pmoles of phosphate incorporated during a 10 min incubation. Controls are performed with appropriate dilutions of dimethylsulfoxide. In a few cases phosphorylation of the substrate is assessed by autoradiography after SDS-PAGE. GSK-3α/β is purified from porcine brain by affinity chromatography on immobilized axin. It is assayed, following a 1/100 dilution in 1 mg BSA/ml 10 mM DTT, with 5 μl 40 μM GS-1 peptide, a specific GSK-3 substrate, (YRRAAVPPSPSLSRHSSPHQSpEDEEE), in buffer A, in the presence of 15 μM [γ-32P] ATP (3,000 Ci/mmol; 1 mCi/ml) in a final volume of 30 μl. After 30 min incubation at 30°C, 25 μl aliquots of supernatant are spotted onto 2.5 × 3 cm pieces of Whatman P81 phosphocellulose paper, and 20 seconds later, the filters are washed five times (for at least 5 min each time) in a solution of 10 ml phosphoric acid/liter of water. The wet filters are counted in the presence of 1 ml ACS scintillation fluid. |
Cell Research | Cell cultures are harvested with 0.05% trypsin and seeded in triplicate at 2 × 104 in 24-well culture plates for 24 h before drug treatment. Culture medium is used for mock treatment. Cells are harvested at the indicated day after treatment, and viable cells are counted using the Vi-cell viability analyze(Only for Reference) |
Synonyms | TAE226 |
Molecular Weight | 468.94 |
Formula | C23H25ClN6O3 |
CAS No. | 761437-28-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 2 years
DMSO: 87 mg/mL (185.5 mM)
Ethanol: <1 mg/mL
H2O: <1 mg/mL
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
NVP-TAE 226 761437-28-9 Angiogenesis Apoptosis Cytoskeletal Signaling Tyrosine Kinase/Adaptors IGF-1R PYK2 FAK c-Met/HGFR inhibit TAE-226 Focal adhesion kinase Insulin Receptor TAE226 Inhibitor PTK2 protein tyrosine kinase 2 Pyk2 NVPTAE 226 Proline-rich tyrosine kinase 2 NVP TAE 226 PTK2 TAE 226 inhibitor