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Embelin

Catalog No. T6485 CAS 550-24-3

Synonyms: NSC 91874, Emberine, Embelic acid

Embelin (Embelic acid), isolated from the Japanese Ardisia herb, is an inhibitor of the X-linked inhibitor of apoptosis (IC50: 4.1 uM).

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Embelin, CAS 550-24-3

Pack Size	Availability	Price/USD
10 mg	In stock	$ 50.00
25 mg	In stock	$ 85.00
50 mg	In stock	$ 160.00
100 mg	In stock	$ 314.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.88%

Purity: 97.07%

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Description	Embelin (Embelic acid), isolated from the Japanese Ardisia herb, is an inhibitor of the X-linked inhibitor of apoptosis (IC50: 4.1 uM).
Targets&IC50	X linked:4.1 μM
In vitro	Embelin from the Japanese Ardisia herb is a small-molecular inhibitor that binds to the XIAP BIR3 domain with which Smac and caspsase-9 bind. Embelin inhibits cell growth of both PC-3 and LNCap cells in a dose-dependent manner, with IC50 values of 3.7 and 5.7 μM. While, the toxicity of Embelin in normal PrEC and in WI-38 cells is much lower with IC50 values of 20.1 μM and 19.3 μM. Treatment of PC-3 cells with 25 and 50 μM of embelin for 48 h, 30% and 75% of cells undergo apoptosis, representing approximately 3-and 9-fold increase as compared to untreated cells. [1] Embelin also potently suppresses the biosynthesis of eicosanoids by selective inhibition of 5-lipoxygenase (5-LO) and Microsomal prostaglandin E2 synthase-1 (mPGES-1) with IC50 = 0.06 and 0.2 mM, respectively. [2]
Kinase Assay	Fluorescence Polarization Competitive Binding Assay: Fluorescence polarization experiments are performed in Dynex 96-well, black, round-bottom plates. A 5 μL sample of Embelin dilutions in DMSO, and preincubated XIAP BIR3 protein (0.06 μM) and the N terminus of a Smac peptide (SM7F) (0.01 μM) in the assay buffer are added to 96-well plates to produce a final volume of 125 μL. For each assay, the bound peptide control containing XIAP BIR3 protein and SM7F (equivalent to 0% inhibition) and free peptide control containing only free SM7F (equivalent to 100% inhibition) were included. The plates were mixed and incubated at room temperature for 3 h to reach equilibrium.
Cell Research	Cell growth is determined by the MTT-based assay using Cell Proliferation Reagent WST-1 according to the manufacturer's instruction. Cells (5000 cells/well) are grown in medium with 10% FBS, and various concentrations of Embelin are added to the cells. Four to five days later, WST-1 is added to each well and incubated for 1-3 h at 37 °C. Absorbance is measured with a plate reader at 450 nm with correction at 650 nm.(Only for Reference)
Source	Natural Products > Rubiaceae > Paederia

Synonyms	NSC 91874, Emberine, Embelic acid
Molecular Weight	294.39
Formula	C17H26O4
CAS No.	550-24-3

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 2.9 mg/mL (10 mM)), Heating is recommended.

DMSO: 29.4 mg/mL (100 mM)

References and Literature

1. Nikolovska-Coleska Z, et al. J Med Chem, 2004, 47(10), 2430-2440. 2. Schaible AM, et al. Biochem Pharmacol, 2013, 86(4), 476-486.

Related compound libraries

This product is contained In the following compound libraries：

Anti-Cancer Active Compound Library Inhibitor Library Anti-Cancer Drug Library Drug Repurposing Compound Library Anti-Cancer Approved Drug Library GPCR Compound Library Drug-Fragment Library RO5 Drug-like Natural Product Library Oxidation-Reduction Compound Library Anti-Cancer Compound Library

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Keywords

Embelin 550-24-3 Apoptosis Autophagy GPCR/G Protein Immunology/Inflammation Metabolism NF-Κb IAP Lipoxygenase NF-κB Prostaglandin Receptor NSC 91874 Nuclear factor-κB carcinoma Nuclear factor-kappaB growth inhibit cancer squamous nonpeptidic cell Emberine Embelic acid death Inhibitor NSC-91874 NSC91874 prostate inhibitor

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