Anti-CD47 Polyclonal Antibody is a Rabbit antibody targeting CD47. Anti-CD47 Polyclonal Antibody can be used in WB,FCM.

IgG

Human,Mouse,Rat (predicted:Rabbit)

1. Blank control: RSC96 (blue), the cells were fixed with 2% paraformaldehyde (10 min).
Isotype Control Antibody: Rabbit Igg (orange);
Secondary Antibody: Goat anti-rabbit IgG-FITC (white blue),
Dilution: 1:100 in 1 X PBS containing 0.5% BSA;
Primary Antibody Dilution: 1 μg in 100 μL 1X PBS containing 0.5% BSA (green).
2. Sample:
Lane 1: Heart (Mouse) Lysate at 40 μg
Lane 2: Kidney (Mouse) Lysate at 40 μg
Primary: Anti-CD47 (TMAB-00375) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
3. Sample:
Lane 1: Lung (Mouse) Lysate at 30 μg
Lane 2: Bone (Mouse) Lysate at 30 μg
Primary: Anti-CD47 (TMAB-00375) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa
4. Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-CD47 antibody (TMAB-00375)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.
5. Blank control: MCF-7. Primary Antibody (green line): Rabbit Anti-CD47 antibody (TMAB-00375)
Dilution: 2 μg/Test;
Secondary Antibody: Goat anti-rabbit IgG-FITC
Dilution: 0.5 μg/Test.
Protocol
The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature.

WB,FCM

WB: 1:500-2000; FCM: 2μg/Test

Polyclonal

KLH conjugated synthetic peptide: human CD47/MER6

Human

CD markers,Adhesion