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Costunolide

Catalog No. T2902   CAS 553-21-9
Synonyms: Costus lactone, NSC 106404, Costunolid, (+)-Costunolide

Costunolide (Costus lactone) has anti-inflammatory and anti-oxidant properties and mediates apoptosis.

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Costunolide Chemical Structure
Costunolide, CAS 553-21-9
Pack Size Availability Price/USD Quantity
5 mg In stock $ 55.00
10 mg In stock $ 79.00
25 mg In stock $ 128.00
50 mg In stock $ 192.00
100 mg In stock $ 285.00
500 mg In stock $ 686.00
1 mL * 10 mM (in DMSO) In stock $ 50.00
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Purity: 100%
Purity: 100%
Purity: 91.75%
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Biological Description
Chemical Properties
Storage & Solubility Information
Description Costunolide (Costus lactone) has anti-inflammatory and anti-oxidant properties and mediates apoptosis.
Targets&IC50 FPTase:20 μM, Telomerase (MCF-7 cells):90 μM, Telomerase (MDA-MB-231 cells):65 μM
In vitro Costunolide reduces neovascularization induced by ethyl ester resin scale plaster in the corneal micropocket model of mice. It inhibits angiogenesis by blocking the vascular endothelial growth factor signaling pathway.
In vivo Costunolide has been shown to inhibit cell proliferation in a dose-dependent manner over a 48-hour treatment in various human tumor cells, including A549, SK-OV-3, SK-MEL-2, XF498, and HCT-15. It induces apoptosis in HL-60 human leukemia cells through ROS-mediated mitochondrial permeability transition and cytochrome C release. Additionally, Costunolide exhibits significant antifungal activity against organisms such as Trichophyton rubrum, T. mentagrophytes, and Microsporum gypseum. The compound also suppresses telomerase activity and cell proliferation in MCF-7 and MDA-MB-231 cells in a concentration and time-dependent manner. Furthermore, Costunolide dose-dependently inhibits the farnesylation of lamin B by farnesyl protein transferase, demonstrating its diverse biochemical impacts.
Kinase Assay Telomerase activity assay: The telomerase activity is measured by the TRAP assay using the TRAPez Telomerase Detection Kit, which includes primers of a 36-bp internal control (IC) for quantifying the amplification of telomerase activity within a linear range close to 2.5 logs. For RNase treatment, 10μL of extract are incubated with 1μg of RNase at 37 °C for 20 minutes. The products of the TRAP assay are resolved by electrophoresis in a nondenaturing12% PAGE in a buffer containing 0.5 × Tris–borate EDTA and detected by autoradiograph. For quantification of TRAP products, the dried gels are exposed to Fuji Imaging Plate at room temperature. Results are corrected for background, and a standard value of 100 is given to the untreated control cell signal. Signal intensities of Costunolide-treated cells are compared to the standard and are expressed as a fraction of the maximum value of 100. [1]
Cell Research 1) Plate 500-10,000 cells in 200 μL media per well in a 96 well plate. Leave 8 wells empty for blank controls. 2) Incubate (37 °C, 5% CO2) overnight to allow the cells to attach to the wells. 3) Add 2 μL of Costunolide dissolved in DMSO to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the samples into the media. 5) Incubate (37 °C, 5% CO2) for 48 hours to allow Costunolide to take effect. 6) Make 2 mL or more of MTT solution per 96 well plate at 5 mg/mL in PBS. Do not make a stock as MTT in solution is not stable long-term. 7) Add 20 μL MTT solution to each well. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the MTT into the media. 8) Incubate (37 °C, 5% CO2) for 1-5 hours to allow the MTT to be metabolized. 9) Dump off the media. (Dry plate on paper towels to remove residue if necessary. 10) Resuspend formazan (MTT metabolic product) in 200 μL DMSO. Place on a shaking table, 150 rpm for 5 minutes, to thoroughly mix the formazan into the solvent. 11) Read optical density at 560 nm and subtract background at 670 nm. Optical density should be directly correlated with cell quantity. (Only for Reference)
Source
Synonyms Costus lactone, NSC 106404, Costunolid, (+)-Costunolide
Molecular Weight 232.32
Formula C15H20O2
CAS No. 553-21-9

Storage

store at low temperature

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 11.6 mg/mL (50 mM)

Ethanol: 11.6 mg/mL (50 mM)

TargetMolReferences and Literature

1. Choi SH, et al, Cancer Lett, 2005, 227(2), 153-162. 2. Park SH, et al, Planta Med, 2001, 67(4), 358-359. 3. Lee MG, et al, Biol Pharm Bull, 2001, 24(3), 303-306. 4. Duripandiyan V, et al, BMC Complement Altern Med, 2012, 12, 13. 5. Jeong SJ, et al, Cancer Lett, 2002, 187(1-2), 129-133.

Related compound libraries

This product is contained In the following compound libraries:
Anti-Inflammatory Traditional Chinese Medicine Compound Library Traditional Mongolian Medicine Compound Library Bioactive Compounds Library Max DNA Damage & Repair Compound Library FDA-Approved & Pharmacopeia Drug Library Selected Plant-Sourced Compound Library Human Endogenous Metabolite Compound Library Plus Apoptosis Compound Library Anti-Aging Compound Library Anti-Cancer Active Compound Library

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Keywords

Costunolide 553-21-9 Apoptosis DNA Damage/DNA Repair Metabolism Endogenous Metabolite Telomerase Inhibitor antioxidants Costus lactone anti-allergic regenerating anticancer antimicrobial antidiabetic anti-inflammatory NSC 106404 growth bone NSC106404 neuroprotective NSC-106404 Costunolid promoting inhibit (+)-Costunolide hair inhibitor

 

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