Home Tools

Col003

Catalog No. T10860 CAS 328565-16-8

Col003 is a selective and potent inhibitor of Hsp47 and competitively binds to the collagen-binding site on Hsp47 (IC50: 1.8 μM). It can be used for the investigation of fibrosis.

All products from TargetMol are for Research Use Only. Not for Human or Veterinary or Therapeutic Use.

Col003, CAS 328565-16-8

Pack Size	Availability	Price/USD
2 mg	In stock	$ 39.00
5 mg	In stock	$ 64.00
10 mg	In stock	$ 109.00
25 mg	In stock	$ 228.00
50 mg	In stock	$ 369.00
100 mg	In stock	$ 571.00
200 mg	In stock	$ 819.00
500 mg	In stock	$ 1,230.00
1 mL * 10 mM (in DMSO)	In stock	$ 64.00

Bulk Inquiry

Select Batch

Purity: 99.03%

Purity: 98.67%

Biological Description

Chemical Properties

Storage & Solubility Information

Description	Col003 is a selective and potent inhibitor of Hsp47 and competitively binds to the collagen-binding site on Hsp47 (IC50: 1.8 μM). It can be used for the investigation of fibrosis.
Targets&IC50	HSP47:1.8 μM
In vitro	A molecule AK778 and its cleavage product Col003 competitively inhibited the interaction and caused the inhibition of collagen secretion by destabilizing the collagen triple helix. Structural information obtained with NMR analysis revealed that Col003 competitively binds to the collagen-binding site on Hsp47. Col003 has an inhibitory effect on the interaction of Hsp47 with collagen in Hsp47 KO / MEFs. Col003 (0.01-100 μM) exhibits dose-dependent effects on the interactions of Hsp47 with collagen (IC50: 1.8 μM for Hsp47). Col003 (100 μM; 20-60 mins) has an inhibitory effect on collagen secretion and accumulation. It inhibits collagen secretion by wild-type MEFs and the secretion is not abolished completely. Col003 (100 μM) degrades α,α′-dipyridyl collagen secretion completely by incubation with trypsin at a high temperature (50 °C for 15 min), but is resistant to trypsin digestion at 37 °C.

Molecular Weight	257.24
Formula	C14H11NO4
CAS No.	328565-16-8

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

DMSO: 25 mg/mL (97.19 mM), Sonication is recommended.

References and Literature

1. Ito S, et al. A small-molecule compound inhibits a collagen-specific molecular chaperone and could represent a potential remedy for fibrosis. J Biol Chem. 2017 Dec 8;292(49):20076-20085.

Citations

1. Wang J, Bai M, Zhang C, et al.Natural compound fraxinellone ameliorates intestinal fibrosis in mice via direct intervention of HSP47-collagen interaction in the epithelium.Acta Pharmacologica Sinica.2023: 1-10.

Related compound libraries

This product is contained In the following compound libraries：

Inhibitor Library Bioactive Compound Library Metabolism Compound Library Anti-Metabolism Disease Compound Library Bioactive Compounds Library Max Anti-Breast Cancer Compound Library Cytoskeletal Signaling Pathway Compound Library Anti-Cancer Compound Library Covalent Inhibitor Library NO PAINS Compound Library

Related Products

Related compounds with same targets

Apoptozole 4beta-Hydroxywithanolide E Gamitrinib TPP hexafluorophosphate HSP90-IN-27 CCT018159 TRC051384 HCl Elesclomol HSP90-IN-19

Dose Conversion

You can also refer to dose conversion for different animals. More

In vivo Formulation Calculator (Clear solution)

Step One: Enter information below

Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step Two: Enter the in vivo formulation

% DMSO+

% +

% Tween 80+

% ddH₂O

%DMSO+

Calculate Reset

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL)，Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.

Method for preparing in vivo formulation：Take μL DMSO master liquid, next add μL PEG300， mix and clarify, next add μL Tween 80，mix and clarify, next add μL ddH₂O, mix and clarify.

Note:

Be sure to add the solvent(s) in order. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Calculator

Molarity Calculator

Dilution Calculator

Reconstitution Calculation

Molecular Weight Calculator

Mass

Concentration

Volume

Molecular Weight

Molarity Calculator allows you to calculate the

Mass of a compound required to prepare a solution of known volume and concentration
Volume of solution required to dissolve a compound of known mass to a desired concentration
Concentration of a solution resulting from a known mass of compound in a specific volume

See Example

An example of a molarity calculation using the molarity calculator
What is the mass of compound required to make a 10 mM stock solution in 10 ml of water given that the molecular weight of the compound is 197.13 g/mol?
Enter 197.13 into the Molecular Weight (MW) box
Enter 10 into the Concentration box and select the correct unit (millimolar)
Enter 10 into the Volume box and select the correct unit (milliliter)
Press calculate
The answer of 19.713 mg appears in the Mass box

Concentration (Start)

Volume (Start)

Concentration (End)

Volume (End)

Calculator the dilution required to prepare a stock solution

Calculate the dilution required to prepare a stock solution
The dilution calculator is a useful tool which allows you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.

See Example

An example of a dilution calculation using the Tocris dilution calculator
What volume of a given 10 mM stock solution is required to make 20ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 ml and V1 is the unknown:
Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
Enter 20 into the Volume (final) box and select the correct unit (milliliter)
Press calculate
The answer of 100 microliter (0.1 ml) appears in the Volume (start) box

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Calculate the volume of solvent required to reconstitute your vial.

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial.
Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Molecule Formula

Molecular Weight g/mol

Enter the chemical formula of a compound to calculate its molar mass and elemental composition

Tip: Chemical formula is case sensitive: C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:
To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.
Definitions of molecular mass, molecular weight, molar mass and molar weight:
Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed n the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

bottom

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.

Keywords

Col003 328565-16-8 Cytoskeletal Signaling Metabolism HSP MEF structure Hsp47 triple helix collagen Col-003 secretion Heat shock proteins Inhibitor Col 003 inhibit interaction inhibitor

Products are chemical reagents for research use only and are not intended for human use. We do not sell to patients.