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Anisomycin

Catalog No. T6758 CAS 22862-76-6

Synonyms: Flagecidin, NSC 76712, Wuningmeisu C

Anisomycin (NSC-76712) is an antibiotic isolated from various Streptomyces species. It interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system.

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Anisomycin, CAS 22862-76-6

Pack Size	Availability	Price/USD
10 mg	In stock	$ 46.00
25 mg	In stock	$ 63.00
50 mg	In stock	$ 90.00
100 mg	In stock	$ 161.00
200 mg	In stock	$ 225.00
1 mL * 10 mM (in DMSO)	In stock	$ 50.00

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Purity: 99.81%

Purity: 99.66%

Purity: 99.11%

Purity: 98.87%

Biological Description

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Description	Anisomycin (NSC-76712) is an antibiotic isolated from various Streptomyces species. It interferes with protein and DNA synthesis by inhibiting peptidyl transferase or the 80S ribosome system.
In vitro	Anisomycin (3 μM) decreases protein synthesis in MDA16 and MDA-MB-468 cells, and reduces colony formation by MDA-MB-468 cells. Anisomycin causes an increase in the number of apoptotic cells in MDA-MB-468 cultures, but not in MDA16 cultures. Anisomycin actives JNK phosphorylation in MDA-MB-468 cells.[2] In U251 and U87 cells, anisomycin?(0.01-8 μM) inhibits the cell growth in time- and concentration-dependent manners with the IC50 (48 h) values of 0.233 and 0.192 μmol/L, respectively. Anisomycin?(4 μM) causes 21.5% and 25.3% of apoptosis proportion in U251 and U87 cells, respectively, and activates p38 MAPK and?JNK, while inactivated ERK1/2. Anisomycin?(4 μM) reduces the level of PP2A/C subunit in a time-dependent manner in U251 and U87 cells.[3] Anisomycin inhibits EAC cell proliferation in concentration-dependent manner.[4]
In vivo	Peritumoral administration of anisomycin (5 mg/kg) significantly suppresses Ehrlich ascites carcinoma (EAC) growth resulting in the survival of approximately 60% of the mice 90 days after EAC inoculation.[4]
Kinase Assay	JNK phosphorylation: 500,000 cells/well are seeded in 6-well plates and incubated overnight. Cells are then incubated for 1 h with test compounds or DMSO as vehicle control (?nal concentration 1% v/v). Puromycin is added (?nal concentration of 18 μM) and cells incubated for a further 10 min to label nascent polypeptide chains. Background labelling is determined by incubating cells without puromycin. Cells are then washed in HBSS, harvested by scraping and centrifuged (300 g, 5 min). Cells are resuspended in 0.5 mL 50 mM DTT containing phosphatase inhibitors and incubated at 95℃ for 10 min. Samples are then snap frozen in liquid nitrogen and stored at -20℃ until blotted. Samples (20–30 μg protein/sample) are blotted onto a PVDF membrane. The membrane is blocked and incubated with anti-phospho-Thr183/Tyr185-JNK antibody overnight at 4℃. Secondary antibodies are used to label the primary antibody and detected using an infrared scanner. The intensity of the ?uorescence signal for anti-phospho-JNK antibody is background corrected and normalized for loading.
Cell Research	For the assay, EAC cells are plated in 96-well plates at a density of 10,000 cells/well/200 μL of medium. The cells are treated with the different concentrations of anisomycin for 48 h. Adriamycin (500 ng/mL) is used as a positive control. 0.5 mg/mL of MTT is added to each well. 4 h later, the formazan product of MTT reduction is dissolved in DMSO, and absorbance is measured at 570 nm using a Model 680 microplate reader.(Only for Reference)

Synonyms	Flagecidin, NSC 76712, Wuningmeisu C
Molecular Weight	265.3
Formula	C14H19NO4
CAS No.	22862-76-6

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 13.3 mg/mL (50 mM)

DMSO: 26.5 mg/mL (100 mM)

References and Literature

1. Iordanov MS, et al. Mol Cell Biol. 1997, 17(6), 3373-3381. 2. Monaqhan D, et al. Biochem Biophys Res Commun. 2014, 443(2), 761-767. 3. Li JY, et al. Acta Pharmacol Sin. 2012, 33(7), 935-940. 4. You P, et al. Oncol Rep. 2013, 29(6), 2227-2236. 5. Chen L, Zhou X, Kong X, et al. The Prognostic Significance of Anisomycin-Activated Phospho-c-Jun NH2-Terminal Kinase (p-JNK) in Predicting Breast Cancer Patients’ Survival Time[J]. Frontiers in Cell and Developmental Biology. 2021, 9: 470. 6. Liu Y J, Chang Y J, Kuo Y T, et al. Targeting β-tubulin/CCT-β complex induces apoptosis and suppresses migration and invasion of highly metastatic lung adenocarcinoma[J]. Carcinogenesis. 2020, 41(5): 699-710.

Citations

1. Chen L, Zhou X, Kong X, et al. The Prognostic Significance of Anisomycin-Activated Phospho-c-Jun NH2-Terminal Kinase (p-JNK) in Predicting Breast Cancer Patients’ Survival Time. Frontiers in Cell and Developmental Biology. 2021 Mar 9;9:656693. doi: 10.3389/fcell.2021.656693. eCollection 2021. 2. Liu Y J, Chang Y J, Kuo Y T, et al. Targeting β-tubulin/CCT-β complex induces apoptosis and suppresses migration and invasion of highly metastatic lung adenocarcinoma. Carcinogenesis. 2020, 41(5): 699-710 3. Zhang L, Liu W, Wu N, et al.Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insect.PLoS pathogens.2023, 19(1): e1011134.

Related compound libraries

This product is contained In the following compound libraries：

Kinase Inhibitor Library Antiparasitic Natural Product Library Microbial Natural Product Library Inhibitor Library Anti-Neurodegenerative Disease Compound Library Bioactive Compound Library Anti-Aging Compound Library RO5 Drug-like Natural Product Library ReFRAME Related Library Anti-Prostate Cancer Compound Library

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Keywords

Anisomycin 22862-76-6 Apoptosis Cell Cycle/Checkpoint DNA Damage/DNA Repair MAPK Microbiology/Virology Antibiotic JNK Antibacterial DNA/RNA Synthesis Bacterial Inhibitor NSC-76712 Flagecidin NSC 76712 inhibit Wuningmeisu C NSC76712 inhibitor

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