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Tyrosine Kinase/Adaptors Trk receptor 7,8-Dihydroxyflavone

7,8-Dihydroxyflavone

Catalog No. T2816   CAS 38183-03-8
Synonyms: 7,8-DHF

7, 8-Dihydroxyflavone (7, 8-DHF) is a naturally-occurring flavone and exist in Tridax procumbens, Godmania aesculifolia, and primula tree leaves.

7,8-Dihydroxyflavone, CAS 38183-03-8
Pack Size Availability Price/USD Quantity
10 mg In stock 38.00
25 mg In stock 55.00
50 mg In stock 77.00
100 mg In stock 100.00
200 mg In stock 130.00
1 mL * 10 mM (in DMSO) In stock 84.00
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Purity 98.37%
Biological Description
Chemical Properties
Storage & Solubility Information
Description 7, 8-Dihydroxyflavone (7, 8-DHF) is a naturally-occurring flavone and exist in Tridax procumbens, Godmania aesculifolia, and primula tree leaves.
Targets&IC50 TrkB
In vitro 7,8-DHF is one of the positive compounds that specifically activate TrkB, but not TrkA or TrkC, at a concentration of 250 nM. In addition to cortical and hippocampal neurons, 7,8-DHF also protects other cell types including the RGC (retinal ganglion cells) and PC12 cells from excitotoxic and oxidative stress-induced apoptosis and cell death. Thus, it has neuroprotective properties[1].
In vivo 7,8-Dihydroxyflavone is a bioavailable chemical that can pass through the BBB to provoke TrkB and its downstream PI3K/Akt and MAPK activation in mouse brain upon intraperitoneal or oral administration. 7,8-DHF promotes the survival and reduces apoptosis in cortical neurons of traumatic brain injury as administration of 7,8-DHF at 3 h post-injury reduces brain tissue damage via the PI3K/Akt pathway. Its treatment does not induce any apparent toxicity in mice and is not toxic to the mice during the chronic treatment. 7,8-DHF displays robust therapeutic efficacy toward Alzheimer's disease and inhibits obesity through activating muscular TrkB[1].
Cell Research
PC12 cells are seeded in 96-well plates at 104/well. After pretreatment with 7,8-DHF (1-25 μM) for 1 h, the cells are exposed to 6-OHDA (100 μM) for subsequent 24 h. The PI3k inhibitor LY294002 or MEK inhibitor PD98059 is added 30 min before 7,8-DHF treatment. At the end of the experiment, PC12 cells are incubated with 20 μl of MTT solution (5 mg/ml in PBS) for 4 h at 37 ◦C. The dark blue formazan product due to the reduction of MTT is dissolved in 150 μl of DMSO, and the absorbance at 570 nm is recorded with a microplate reader. The viability is expressed as the percentage of the untreated control cells. (Only for Reference)
Cell lines: Rat pheochromocytoma (PC12) cells
Synonyms 7,8-DHF
Purity 98.37%
Molecular Weight 254.24
Formula C15H10O4
CAS No. 38183-03-8

Storage

0-4℃ for short term (days to weeks), or -20℃ for long term (months).

Solubility Information

DMSO: 47 mg/mL (184.9 mM)

Ethanol: 1 mg/mL (3.93 mM)

Water: <1 mg/mL

( < 1 mg/ml refers to the product slightly soluble or insoluble )

Citations

References and Literature
1. Liu C, et al. Transl Neurodegener. 2016, 5:2. 2. Bollen E, et al. Behav Brain Res. 2013, 257:8-12. 3. Han XH, et al. Neurosci Lett. 2014, 581:85-8.

Related compound libraries

This product is contained In the following compound libraries:
Bioactive Compound Library Inhibitor Library Natural Compound Library for HTS Anti-cancer Compound Library Apoptosis Compound Library Tyrosine kinase inhibitor library Selected Plant-sourced Compound Library Kinase Inhibitor Library Polyphenolic Natural Compound Library Anti-obesity Compound Library Anti-aging Compound Library Antitumor Natural Products Library Target-Focused Phenotypic Screening Library Flavonoids natural compound library Ferroptosis Compound Library Neurodegenerative disease-related Compound Library Anti-Lung Cancer Compound Library Traditional Chinese Medicine Monomer Library Anti-Alzheimer's Disease Compound Library

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