Powder: -20°C for 3 years
In solvent: -80°C for 2 years
2-Methoxyestradiol is an orally bioavailable estradiol metabolite with potential antineoplastic activity. 2-Methoxyestradiol inhibits angiogenesis by reducing endothelial cell proliferation and inducing endothelial cell apoptosis. This agent also inhibits tumor cell growth by binding to tubulin, resulting in antimitotic activity, and by inducing caspase activation, resulting in cell cycle arrest in the G2 phase, DNA fragmentation, and apoptosis.
Description | 2-Methoxyestradiol is an orally bioavailable estradiol metabolite with potential antineoplastic activity. 2-Methoxyestradiol inhibits angiogenesis by reducing endothelial cell proliferation and inducing endothelial cell apoptosis. This agent also inhibits tumor cell growth by binding to tubulin, resulting in antimitotic activity, and by inducing caspase activation, resulting in cell cycle arrest in the G2 phase, DNA fragmentation, and apoptosis. |
Kinase Assay | Microtubule depolymerizing activity: The effects of 2-Methoxyestradiol on cellular microtubule depolymerization are determined by indirect immunofluorescence techniques in rat aortic smooth muscle A-10 cells. Microtubules are visualized using a β-tubulin antibody. Three viewers determines the percent microtubule loss for each treatment concentration. The data are averaged and plotted as percent microtubule loss versus drug concentration and the EC50s for microtubule depolymerization calculated from the log dose–response curves. |
Cell Research | The sulforhodamine B (SRB) assay is used to evaluate the antiproliferative activity of 2-Methoxyestradiol in the MDA-MB-435 and SK-OV-3 cell lines. Cells a plated into 96-well plates and allowed to grow and attach for 24 hours followed by addition of 2-Methoxyestradiol or vehicle controls. The cells are incubated with drugs for 48 hours and then the cellular protein is fixed, stained, and concentration determined by absorbance at 560 nm. Log dose–response curves are constructed for each experiment and the IC50 for inhibition of proliferation determined.(Only for Reference) |
Synonyms | 2-MeOE2, 2-ME2, NSC-659853, 2-甲氧雌二醇, 二甲氧基雌二醇 |
Molecular Weight | 302.41 |
Formula | C19H26O3 |
CAS No. | 362-07-2 |
Powder: -20°C for 3 years
In solvent: -80°C for 2 years
DMSO: 15.1 mg/mL (50 mM)
( < 1 mg/ml refers to the product slightly soluble or insoluble )
You can also refer to dose conversion for different animals. More
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2-Methoxyestradiol 362-07-2 NF-Kb信号通路 表观遗传 代谢 凋亡 免疫与炎症 细胞骨架 血管生成 自噬 Apoptosis Autophagy Endogenous Metabolite HIF Microtubule Associated Reactive Oxygen Species NSC 659853 NSC659853 NSC-659853 2Methoxyestradiol 2-MeOE2 Microtubule/Tubulin Inhibitor 2 Methoxyestradiol 2-ME2 inhibit inhibitor