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2,5-Dihydroxybenzoic acid

Name: 2,5-Dihydroxybenzoic acid
Brand: TargetMol
SKU: T2857
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T2857Cas No. 490-79-9

Alias Phloretate, Hydroquinonecarboxylic acid, Gentisic acid, Gentianic acid, Carboxyhydroquinone, 5-Hydroxysalicylic acid

2,5-Dihydroxybenzoic acid (Gentisic acid) is an active metabolite of salicylic acid degradation with a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.

2,5-Dihydroxybenzoic acid

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Purity: 99.63%

Catalog No. T2857Alias Phloretate, Hydroquinonecarboxylic acid, Gentisic acid, Gentianic acid, Carboxyhydroquinone, 5-Hydroxysalicylic acidCas No. 490-79-9

Pack Size	Price	USA Warehouse	Global Warehouse
50 mg	$30	In Stock	In Stock
100 mg	$40	In Stock	In Stock
500 mg	$81	-	In Stock
1 g	$123	-	In Stock
1 mL x 10 mM (in DMSO)	$44	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Batch Information

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Purity:99.63%

Appearance:Solid

Color:White

Resource Download

COA LCMS HNMR

Product Introduction

2,5-Dihydroxybenzoic acid AI Summary

2,5-Dihydroxybenzoic acid exhibits a wide range of bioactivities and inhibitory effects on various enzymes and receptors. It shows significant inhibitory activity against pieris rapae Phenoloxidase (IC50 values of 32.5 μM and 30.9 μM), 15-lipoxygenase (49.9% inhibition), and human recombinant 5-lipoxygenase (86% inhibition at 1mM). It also effectively inhibits mushroom tyrosinase (96% inhibition at 1mM) and demonstrates moderate inhibition of human recombinant MMP9 (40%) and MMP1 (13%). Additionally, 2,5-Dihydroxybenzoic acid has antioxidant properties, with an IC50 value of 4.26 μM for scavenging DPPH radicals. The compound inhibits various human carbonic anhydrase isoforms, particularly carbonic anhydrase 2 and 1 (Ki values ranging from 4.1 μM to 73 μM). It shows potent inhibition of sodium fluorescein uptake in OATP1B1 and OATP1B3 transporters with high inhibition rates (84.57% and 104.03% respectively, at 10 μM). Moreover, 2,5-Dihydroxybenzoic acid exhibits bioactivities as an inhibitor of HP1-beta chromodomain interactions (IC50 = 100 μM), Polymerase Iota (IC50 = 89.1 μM), Selenoprotein Thioredoxin Reductase 1 (IC50 = 50.1 μM), and PTP1B (IC50 > 100 μM). It also inhibits human PTGS2 (IC50 = 5.8 μM) and rat Ptgs1 (IC50 = 17.999 μM), and displays antagonist activity at human CHRM1 and TBXA2R. Other reported activities include a low octanol-aqueous phase distribution coefficient (log D = -3.11) and moderate log P (1.72), as well as specific interaction with carbonic anhydrases from various species. However, it shows no significant trypanosomicidal activity against Trypanosoma cruzi or cytotoxicity against mouse cells. Overall, 2,5-Dihydroxybenzoic acid demonstrates diverse bioactivity, particularly notable for its enzyme inhibition, antioxidant properties, and receptor interactions, although its potency varies across different targets..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	2,5-Dihydroxybenzoic acid (Gentisic acid) is an active metabolite of salicylic acid degradation with a broad spectrum of biological activity, such as anti-inflammatory, antirheumatic and antioxidant properties.
Synonyms	Phloretate, Hydroquinonecarboxylic acid, Gentisic acid, Gentianic acid, Carboxyhydroquinone, 5-Hydroxysalicylic acid

Chemical Properties

Molecular Weight	154.12
Formula	C₇H₆O₄
Cas No.	490-79-9
Smiles	OC(=O)C1=C(O)C=CC(O)=C1
Relative Density.	1.3725 g/cm3 (Estimated)

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 60 mg/mL (389.31 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (12.98 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	6.4885 mL	32.4423 mL	64.8845 mL	324.4225 mL
5 mM	1.2977 mL	6.4885 mL	12.9769 mL	64.8845 mL
10 mM	0.6488 mL	3.2442 mL	6.4885 mL	32.4423 mL
20 mM	0.3244 mL	1.6221 mL	3.2442 mL	16.2211 mL
50 mM	0.1298 mL	0.6488 mL	1.2977 mL	6.4885 mL
100 mM	0.0649 mL	0.3244 mL	0.6488 mL	3.2442 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc