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1400W dihydrochloride

Name: 1400W dihydrochloride
Brand: TargetMol
SKU: T3491
Price: 43 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T3491Cas No. 214358-33-5

Alias N-(3-(Aminomethyl)benzyl)acetamidine, 1400W 2HCl

1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).

1400W dihydrochloride

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Purity: 99.94%

Catalog No. T3491Alias N-(3-(Aminomethyl)benzyl)acetamidine, 1400W 2HClCas No. 214358-33-5

1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).

Pack Size	Price	USA Warehouse	Global Warehouse
5 mg	$43	In Stock	In Stock
10 mg	$68	In Stock	In Stock
25 mg	$127	In Stock	In Stock
50 mg	$231	In Stock	In Stock
100 mg	$393	In Stock	In Stock
200 mg	$511	In Stock	In Stock
500 mg	$829	In Stock	In Stock
1 mL x 10 mM (in DMSO)	$48	In Stock	In Stock

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In Stock Estimated shipping dateUSA Warehouse[1-2 days] Global Warehouse[5-7 days]

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

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Purity:99.94%

Color:White

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COA LCMS HNMR

Product Introduction

Bioactivity

Description	1400W dihydrochloride (N-(3-(Aminomethyl)benzyl)acetamidine) is a highly effective and specific inhibitor of inducible NOS2 (iNOS).
Targets&IC50	nNOS:2 μM(Ki), iNOS:<7 nM(Kd), eNOS:50 μM(Ki)
In vitro	1400W is either an irreversible inhibitor or an extremely slowly reversible inhibitor of human iNOS. Inhibition of human iNOS by 1400W is time-dependent. 1400W is competitive with L-arginine. 1400W is not a substrate for iNOS[1].
In vivo	1400W selectively prevents microvasculature injury in rats. 1400W is greater than 50-fold more potent against iNOS than eNOS in a rat model of endotoxin-induced vascular injury. Moreover, 1400W also dose-dependently reduces LPS-induced vascular leakage associated with iNOS induction in the colon, lung, liver, kidney, and heart. The maximal protection is close to 100% for all organs except the kidney (kidney:54%)[1]. 1400W has an ameliorative effect on both oxidative and nitrosative stress in the kidneys against renal I/R injury in rats[2]. Treatment with 1400W can reduce the rate of growth of solid tumors in mice[4].
Cell Research	RAW264.7 cells are treated with LPS/IFNγ for 16 h and GAPDH-p300 binding is abolished by the iNOS inhibitor 1400W (100 μM). Cell lysates are immunoprecipitated with an anti-p300 antibody and the immunoprecipitates are analysed by western blotting with an anti-GAPDH antibody. (Only for Reference)
Synonyms	N-(3-(Aminomethyl)benzyl)acetamidine, 1400W 2HCl

Chemical Properties

Molecular Weight	250.17
Formula	C₁₀H₁₇Cl₂N₃
Cas No.	214358-33-5
Smiles	Cl.Cl.CC(=N)NCc1cccc(CN)c1
Relative Density.	no data available

Storage & Solubility Information

Storage

In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

H2O: 139.9 mM, Sonication is recommended.

DMSO: 255 mg/mL (1019.31 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 2 mg/mL (7.99 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

H2O/DMSO

	1mg	5mg	10mg	50mg
1 mM	3.9973 mL	19.9864 mL	39.9728 mL	199.8641 mL
5 mM	0.7995 mL	3.9973 mL	7.9946 mL	39.9728 mL
10 mM	0.3997 mL	1.9986 mL	3.9973 mL	19.9864 mL
20 mM	0.1999 mL	0.9993 mL	1.9986 mL	9.9932 mL
50 mM	0.0799 mL	0.3997 mL	0.7995 mL	3.9973 mL
100 mM	0.0400 mL	0.1999 mL	0.3997 mL	1.9986 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the stock solution preparation method and in vivo formula preparation method:

For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, TargetMol | Animal experiments

and a total of 10 animals are to be administered, using a formulation of TargetMol | reagent

10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH₂O , the resulting working solution concentration would be 2 mg/mL.

Stock Solution Preparation:

Dissolve 2 mg of the compound in 100 μL DMSO TargetMol | reagent to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.

Preparation of the In Vivo Formulation:

1) Add 100 μL of the DMSO TargetMol | reagent stock solution to 400 μL PEG300 and mix thoroughly until the solution becomes clear.

2) Add 50 μL Tween 80 and mix well until fully clarified.

3) Add 450 μL Saline,PBS or ddH₂O TargetMol | reagent and mix thoroughly until a homogeneous solution is obtained.

This example is provided solely to demonstrate the use of the In Vivo Formulation Calculator and does not constitute a recommended formulation for any specific compound. Please select an appropriate dissolution and formulation strategy based on your experimental model and route of administration.

All co-solvents required for this protocol, includingDMSO, PEG300/PEG400, Tween 80, SBE-β-CD, and Corn oil, are available for purchase on the TargetMol website.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc