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(20S)-Protopanaxadiol

(20S)-Protopanaxadiol
(20S)-Protopanaxadiol (20-Epiprotopanaxadiol) (20-Epiprotopanaxadiol), an apoptosis inducer, is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin.
Catalog No. T2799Cas No. 30636-90-9
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Purity:98.91%
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(20S)-Protopanaxadiol

Purity: 98.91%
Catalog No. T2799Alias 20-Epiprotopanaxadiol, 20(S)-APPDCas No. 30636-90-9

(20S)-Protopanaxadiol (20-Epiprotopanaxadiol) (20-Epiprotopanaxadiol), an apoptosis inducer, is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin.
All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.
Pack SizePriceAvailabilityQuantity
5 mg$45In Stock
10 mg$64In Stock
50 mg$243In Stock
100 mg$366In Stock
1 mL x 10 mM (in DMSO)$46In Stock
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Product Introduction

Bioactivity
Description
(20S)-Protopanaxadiol (20-Epiprotopanaxadiol) (20-Epiprotopanaxadiol), an apoptosis inducer, is an aglycon metabolic derivative of the protopanaxadiol-type ginseng saponin.
In vitro
Concentrations causing growth inhibition of 50% of cells (LC50) for the compounds (20S)-Protopanaxadiol is 23 μg/ml, for Int-407 cells. The the LC50 for (20S)-Protopanaxadiol is determined to be 24 μg/ml in Caco-2 cells. In the Int-407 cell line, (20S)-Protopanaxadiol produces great percentage of sub-G1 (apoptotic) cells(The Int-407 cell line is a human embryonic nonmalignant cell line originating from fetal tissue of approximately 2 months gestation, whereas Caco-2 is a human colon adenocarcinoma cell line). This response is attributed to differences in individual ginsenoside glycosylation and, thus, the hydrophobic-hydrophilic balance [2]. Incubation with (20S)-Protopanaxadiol also significantly reduces the viability of U251-MG and U87-MG cells in a dose- and time-dependent manner. The cytotoxic effect of (20S)-Protopanaxadiol is accompanied by reduced expression of cell adhesion proteins, including N-cadherin and integrin β1, which leads to reduced phosphorylation of focal adhesion kinase. Furthermore, incubation with (20S)-Protopanaxadiol reduces the expression of cyclin D1 and subsequently induces cell-cycle arrest at the G1 phase[3].
In vivo
Treatment with (20S)-Protopanaxadiol and PPT prior to immobilization stress increase the time spent in open arms and open arm entries in the elevated plus-maze (EPM) test. Treatment with (20S)-Protopanaxadiol potently suppresses immobilization stress-induced serum levels of corticosterone and interleukin (IL)-6 by the enzyme-linked immunosorbent assay. (20S)-Protopanaxadiol and PPT may exhibit the anxiolytic effect via γ-aminobutyrateA (GABAA) receptor(s) and serotonergic receptor(s), respectively, and (20S)-Protopanaxadiol may have an anti-inflammatory effect that is more potent than that of PPT[1].
Cell Research
Int-407 and Caco-2 cells are seeded at a concentration of 1×106 cells/mL in 24-well plates in separate experiments. Test ginsenosides (PPD, PPT, Rh2) are added to wells to the LC50 concentration determined previously from the MTT assays. The ginsenoside concentrations used for Int-407 cells are 23, 26, and 53 μg/mL, respectively, for PPD, PPT, and Rh2. In a similar experiment using Caco-2 cells, 24 μg/mL for both PPD and PPT and 55 μg/mL for Rh2 is used. Untreated cells represented the control. Cells are incubated at 37℃ in a 5% CO2 humidified incubator for 24, 48, and 72 h. Cell-free supernatant are obtained by centrifugation(400 g) for 10 min, and the lactate dehydrogenase (LDH) assay is conducted as previously reported.(Only for Reference)
Alias20-Epiprotopanaxadiol, 20(S)-APPD
Chemical Properties
Molecular Weight460.73
FormulaC30H52O3
Cas No.30636-90-9
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
Ethanol: 31 mg/mL (67.3 mM)
DMSO: 85 mg/mL (184.6 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Solution Preparation Table
Ethanol/DMSO
1mg5mg10mg50mg
1 mM2.1705 mL10.8523 mL21.7047 mL108.5234 mL
5 mM0.4341 mL2.1705 mL4.3409 mL21.7047 mL
10 mM0.2170 mL1.0852 mL2.1705 mL10.8523 mL
20 mM0.1085 mL0.5426 mL1.0852 mL5.4262 mL
50 mM0.0434 mL0.2170 mL0.4341 mL2.1705 mL
DMSO
1mg5mg10mg50mg
100 mM0.0217 mL0.1085 mL0.2170 mL1.0852 mL

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