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Rhosin hydrochloride

Name: Rhosin hydrochloride
Brand: TargetMol
SKU: T16745
Price: 162 USD
Availability: InStock
Rating: 5 (10 reviews)

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Catalog No. T16745Cas No. 1281870-42-5

Rhosin hydrochloride is a specific inhibitor of the RhoA subfamily Rho GTPases and inhibits the RhoA-GEF interaction. Rhosin hydrochloride can significantly induce cell apoptosis without affecting cell cycle progression. [1]

Rhosin hydrochloride

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Purity: 98.92%

Catalog No. T16745Cas No. 1281870-42-5

Pack Size	Price	Availability
1 mg	$162	In Stock
2 mg	$229	In Stock
5 mg	$347	In Stock
10 mg	$497	In Stock
25 mg	$793	In Stock
50 mg	$1,080	In Stock
100 mg	$1,450	In Stock
1 mL x 10 mM (in DMSO)	$382	In Stock

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Batch Information

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Purity:98.92%

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COA HNMR HPLC

Product Introduction

Bioactivity

Description	Rhosin hydrochloride is a specific inhibitor of the RhoA subfamily Rho GTPases and inhibits the RhoA-GEF interaction. Rhosin hydrochloride can significantly induce cell apoptosis without affecting cell cycle progression. [1]
Targets&IC50	RhoA:(kd)0.4 uM
In vitro	METHODS: To investigate the cytotoxic effects of Rhosin hydrochloride on B16BL6 and 4T1 cells, cell viability was assessed by treating cells with 1-100 μM Rhosin hydrochloride. RESULTS Rhosin hydrochloride at a concentration of 100 μM induced cell death in B16BL6 and 4T1 cells. [1]
In vivo	METHODS: B16BL6 cells and 4T1 cells were injected into the tail vein of syngeneic C57BL/6J mice and Balb/c mice. Mice were treated with 10 or 30 mg/kg of Rhosin hydrochloride daily from day 1 to day 14. RESULTS The number of lung metastatic nodules in B16BL6 and 4T1 cells was reduced in a dose-dependent manner after administration of Rhosin hydrochloride, and Rhosin hydrochloride inhibited the metastasis of 4T1-luc tumor cells to the lung region, which could be confirmed by the reduction of photon flux. [1]

Chemical Properties

Molecular Weight	431.318
Formula	C₂₀H₂₀Cl₂N₆O
Cas No.	1281870-42-5
Smiles	Cl.N[C@H](Cc1c[nH]c2ccccc12)C(=O)N\N=C\c1ccc2nccnc2c1
Relative Density.	no data available

Storage & Solubility Information

Storage

store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.

Solubility Information

DMSO: 100 mg/mL (253.25 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (9.27 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.3185 mL	11.5924 mL	23.1847 mL	115.9237 mL
5 mM	0.4637 mL	2.3185 mL	4.6369 mL	23.1847 mL
10 mM	0.2318 mL	1.1592 mL	2.3185 mL	11.5924 mL
20 mM	0.1159 mL	0.5796 mL	1.1592 mL	5.7962 mL
50 mM	0.0464 mL	0.2318 mL	0.4637 mL	2.3185 mL
100 mM	0.0232 mL	0.1159 mL	0.2318 mL	1.1592 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% Saline/PBS/ddH₂O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLSaline/PBS/ddH₂O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

All types of co-solvents required for the protocol, such asDMSO, PEG300/ PEG400, Tween 80, SBE-β-CD, corn oil are available for purchase on the TargetMol website with a simple click.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc